EBK EXPERIMENTAL ORGANIC CHEMISTRY: A M
EBK EXPERIMENTAL ORGANIC CHEMISTRY: A M
6th Edition
ISBN: 9781305687875
Author: Gilbert
Publisher: CENGAGE LEARNING - CONSIGNMENT
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Chapter 6.3, Problem 9E
Interpretation Introduction

Interpretation: Two major differences between TLC and column chromatography should be stated.

Concept introduction: Thin Layer Chromatography is a sensitive chromatographic technique which is used to analyze small quantities of the samples by solid-liquid adsorption method. Rectangular glass plate is coated with an adsorbent; sample to be analyzed is run on the plate in the developing chamber with the mobile phase to give spots of the analyzed sample.

Column chromatography is a large scale solid-liquid adsorption chromatographic technique that can separate multigram amounts of mixtures. This is a preparative version of thin layer chromatography that permits separation and isolation of products by collection of eluted fractions.

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