The reason for the streak formation on silica gel TLC plates by carboxylic acids and addition of acetic acid to reduce the streaking should be explained. Concept Introduction : Thin Layer Chromatography is a sensitive chromatographic technique which used to analyze small quantities of the samples by solid-liquid adsorption method. Rectangular glass plate is coated with an adsorbent, sample to be analyzed is run on the plate in the developing chamber with the mobile phase to give spots of the analyzed sample.

Question

A tissue plasminogen activator (T PA) opthalmic solution is prepared to contain 25 mg / 100 mL. A.Calculate the percent concentration of TPA in the solution. B.What volume of a solution containing TPA , 50 mg/ 50 mL , should be used to prepare each 100 mL of opthalmic solution?

Answer 1

Question

Definition Definition Class of organic compounds that contain a carboxyl group ( - COOH ) and has a general formula R - COOH or R - CO 2 H , where R refers to the alkyl, alkenyl, aryl, or other groups. They can undergo different chemical reactions, such as acid-base reactions, esterification, and oxidation. These are essential components of living organisms, playing important roles in metabolic processes, signaling, and as pharmaceuticals.

Chapter 6.2, Problem 9E

(a)

Interpretation Introduction

Interpretation:

The reason for the streak formation on silica gel TLC plates by carboxylic acids and addition of acetic acid to reduce the streaking should be explained.

Concept Introduction:

Thin Layer Chromatography is a sensitive chromatographic technique which used to analyze small quantities of the samples by solid-liquid adsorption method. Rectangular glass plate is coated with an adsorbent, sample to be analyzed is run on the plate in the developing chamber with the mobile phase to give spots of the analyzed sample.

(b)

Interpretation Introduction

Interpretation:

The chemical component which has to use to reduce the streaking on the TLC caused by the amine should be identified.

Concept Introduction:

Thin Layer Chromatography is a sensitive chromatographic technique which used to analyze small quantities of the samples by solid-liquid adsorption method. Rectangular glass plate is coated with a adsorbent, sample to be analyzed is run on the plate in the developing chamber with the mobile phase to give spots of the analyzed sample.

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A tissue plasminogen activator (T PA) opthalmic solution is prepared to contain 25 mg / 100 mL. A.Calculate the percent concentration of TPA in the solution. B.What volume of a solution containing TPA , 50 mg/ 50 mL , should be used to prepare each 100 mL of opthalmic solution?

What compounds present in a sample that can cause error in the analysis and alter desired results? A. Matrix B. Selectivity C. Analyte D. Interference

2. When separating acetone (an organic liquid) from LiBr (a solid impurity), which would be the most convenient means of separation? a. steam distillation b. column chromatography c. fractional distillation d. simple distillation Your answer:

Answer 2

Question

Definition Definition Class of organic compounds that contain a carboxyl group ( - COOH ) and has a general formula R - COOH or R - CO 2 H , where R refers to the alkyl, alkenyl, aryl, or other groups. They can undergo different chemical reactions, such as acid-base reactions, esterification, and oxidation. These are essential components of living organisms, playing important roles in metabolic processes, signaling, and as pharmaceuticals.

Chapter 6.2, Problem 9E

(a)

Interpretation Introduction

Interpretation:

The reason for the streak formation on silica gel TLC plates by carboxylic acids and addition of acetic acid to reduce the streaking should be explained.

Concept Introduction:

Thin Layer Chromatography is a sensitive chromatographic technique which used to analyze small quantities of the samples by solid-liquid adsorption method. Rectangular glass plate is coated with an adsorbent, sample to be analyzed is run on the plate in the developing chamber with the mobile phase to give spots of the analyzed sample.

(b)

Interpretation Introduction

Interpretation:

The chemical component which has to use to reduce the streaking on the TLC caused by the amine should be identified.

Concept Introduction:

Thin Layer Chromatography is a sensitive chromatographic technique which used to analyze small quantities of the samples by solid-liquid adsorption method. Rectangular glass plate is coated with a adsorbent, sample to be analyzed is run on the plate in the developing chamber with the mobile phase to give spots of the analyzed sample.

Expert Solution & Answer

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Answer 3

Question

Definition Definition Class of organic compounds that contain a carboxyl group ( - COOH ) and has a general formula R - COOH or R - CO 2 H , where R refers to the alkyl, alkenyl, aryl, or other groups. They can undergo different chemical reactions, such as acid-base reactions, esterification, and oxidation. These are essential components of living organisms, playing important roles in metabolic processes, signaling, and as pharmaceuticals.

Chapter 6.2, Problem 9E

(a)

Interpretation Introduction

Interpretation:

The reason for the streak formation on silica gel TLC plates by carboxylic acids and addition of acetic acid to reduce the streaking should be explained.

Concept Introduction:

Thin Layer Chromatography is a sensitive chromatographic technique which used to analyze small quantities of the samples by solid-liquid adsorption method. Rectangular glass plate is coated with an adsorbent, sample to be analyzed is run on the plate in the developing chamber with the mobile phase to give spots of the analyzed sample.

(b)

Interpretation Introduction

Interpretation:

The chemical component which has to use to reduce the streaking on the TLC caused by the amine should be identified.

Concept Introduction:

Thin Layer Chromatography is a sensitive chromatographic technique which used to analyze small quantities of the samples by solid-liquid adsorption method. Rectangular glass plate is coated with a adsorbent, sample to be analyzed is run on the plate in the developing chamber with the mobile phase to give spots of the analyzed sample.

Expert Solution & Answer

Trending nowThis is a popular solution!

See solution

Check out sample textbook solution

Answer 4

Question

Definition Definition Class of organic compounds that contain a carboxyl group ( - COOH ) and has a general formula R - COOH or R - CO 2 H , where R refers to the alkyl, alkenyl, aryl, or other groups. They can undergo different chemical reactions, such as acid-base reactions, esterification, and oxidation. These are essential components of living organisms, playing important roles in metabolic processes, signaling, and as pharmaceuticals.

Chapter 6.2, Problem 9E

(a)

Interpretation Introduction