EBK EXPERIMENTAL ORGANIC CHEMISTRY: A M
6th Edition
ISBN: 9781305687875
Author: Gilbert
Publisher: CENGAGE LEARNING - CONSIGNMENT
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Question
Chapter 6.2, Problem 2E
Interpretation Introduction
Interpretation:The reason for a mixture containing a highly sensitive component to acidic conditions to be subjected to a TLC analysis in which silica gel serves as the stationary phase should be explained.
Concept introduction:Thin Layer Chromatography is a sensitive chromatographic technique which used to analyze small quantities of the samples by solid-liquid adsorption method. Rectangular glass plate is coated with an adsorbent; sample to be analyzed is run on the plate in the developing chamber with the mobile phase to give spots of the analyzed sample.
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Students have asked these similar questions
In color chromatography of plant pigments, what complications would a dried-out column (solvent level is below the top of the silica) introduce to the elution and isolation of pigments?
More polar molecules move more slowly through silica than less polar molecules. Why is silica gel chromatography a good method of separating methyl orange from biphenyl?
Column chromatography is also very useful for separating mixtures of organic compounds that are not coloured. Briefly explain how you could use column chromatography for the separation of a colourless mixture, and how you could identify your separated compounds.
Chapter 6 Solutions
EBK EXPERIMENTAL ORGANIC CHEMISTRY: A M
Ch. 6.2 - Prob. 1ECh. 6.2 - Prob. 2ECh. 6.2 - Prob. 3ECh. 6.2 - Prob. 4ECh. 6.2 - Prob. 5ECh. 6.2 - Prob. 6ECh. 6.2 - Prob. 7ECh. 6.2 - Prob. 8ECh. 6.2 - Prob. 9ECh. 6.3 - Prob. 1E
Ch. 6.3 - Prob. 2ECh. 6.3 - Prob. 3ECh. 6.3 - Prob. 4ECh. 6.3 - Prob. 5ECh. 6.3 - Prob. 6ECh. 6.3 - Prob. 7ECh. 6.3 - Prob. 8ECh. 6.3 - Prob. 9ECh. 6.3 - Prob. 10ECh. 6.3 - Prob. 11ECh. 6.3 - Prob. 12ECh. 6.3 - Prob. 13ECh. 6.3 - Prob. 14ECh. 6.3 - Prob. 15ECh. 6.3 - Prob. 16ECh. 6.3 - Prob. 17ECh. 6.3 - Prob. 18ECh. 6.3 - Prob. 19ECh. 6.3 - Prob. 20ECh. 6.4 - Prob. 1ECh. 6.4 - Prob. 2ECh. 6.4 - Prob. 3ECh. 6.4 - Prob. 4ECh. 6.4 - Prob. 5E
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- Is thin-layer chromatography a type of normal-phase chromatography or a type of reverse-phase chromatography? Define these two classifications.arrow_forwardTLC was used to compare the solubility of two compounds A and B in a solvent system (acetic acid, methanol, H2O). The distances that solvent has moved in the silica gel TLC was 12 cm. and for the compounds were 2 and 8 cm respectively. (a) Calculate the Rf values and which compound is more soluble and which one is more polar. (b) What are the factors affecting the Rf valuearrow_forwardGel permeation chromatography depends on the degree of species segregation of a samplearrow_forward
- A series of nonpolar aromatic compounds were separated by isocratic HPLC on a nonpolar stationary phase containing octadecyl groups [−(CH2)17CH3] covalently attached to silica particles. The mobile phase was 60/40 methanol/water. How would the retention times be affected if 90/10 methanol/water were used instead and why? What type of chromatography?arrow_forwardPredict the order of elution for the silica gel adsorption TLC separation of the mixture containing aspirin, acetaminophen and caffeine using methylene chloride solvent. Explain.arrow_forwardWhat kind of information does a mass spectrometer detector give in gas chromatography that is useful for qualitative analysis? For quantitative analysis?arrow_forward
- discuss the separation of bisphenol z from phenol on the tlc plate in terms of solubility and bondingarrow_forwardIn silica gel chromatography, which compounds elute first? A-The least polar B-Those with ionic functional groups C-Those with the lowest boiling point D-Those with the highest molecular weight E-The most polararrow_forwardWhich statements are true for molecular exclusion chromatography?I Molecular exclusion chromatography separates on the basis of size.II Small molecules can fit in the pores of the stationary phase and effectively pass through a larger volume, and elute last.III The pores in the stationary phase are too small for large molecules to pass through, so large molecules elute first.IV Molecular exclusion chromatography with a hydrophilic stationary phase and an aqueous solvent is called gel permeation chromatography. A. I, II, III B. III and IV C. II only D. I and II only E. I, III, and IVarrow_forward
- In order to obtain better separation of the mixture, the above analysis was repeated using an HPLC technique. The retention times of compounds A and B were found to be 15.25 and 16.80 min, respectively, on a 40-cm packed column. An unretained species passed through the column in 1.25 min. The peak base width for A and B are 1.15 and 1.27 min, respectively. Calculate: i) The column resolution. ii) The average number of plates in the column. iii) The plate height. iv) The length of column required to achieve a resolution of 1.5.arrow_forwardDescribe the mechanism that allows analites to be separated in:ion-exchange chromatographyarrow_forwardDuring a Thin layer chromatography (TLC) analysis of crystallized napthalene, What would be the effect of adding acetic acid to the mixture in regards to the separation/spot shape?arrow_forward
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