Chapter 6, Problem 67TQ

Summary Introduction

To review:

Information that can be determined by the turnover number ( $k_{cat}$ ) and Michaelis–Menten constant ( $K_m$ ) of enzyme catalase and carbonic anhydrase. For catalase: $K_m$= 25Mm and $k_{cat}$ = $\text{4}{\text{.0×10}}^{\text{7}}{\text{s}}^{\text{-1}}$, whereas, for carbonic anhydrase: $K_m$ =26mM (mill molar) and $k_{cat}$ = $\text{4}{\text{.0×10}}^{\text{5}}{\text{s}}^{\text{-1}}$ (per second).

Introduction:

Michaelis–Menten constant ( $K_m$ ) is defined as the concentration of substrate at which half of the maximum velocity is attained in an enzyme-catalyzed reaction. Turnover number ( $k_{cat}$ ) is equal toV_max/E_t, where V_max is the maximum velocity and E_t is the total enzyme concentration.