Concept explainers
Interpretation:
From the given table, the amino acid sequence that would be an effective reagent for sequencing the protein with chymotrypsin is to be identified and the reason for its effectiveness is to be explained.
Concept introduction:
Before the sequencing experiment of the proteins is carried out, the peptides are initially converted into small length peptides by the action of certain chemicals or enzymes. These enzymes cut the proteins at specific points, to yield smaller fragments of peptides. Examples of such chemicals or enzymes are trypsin, chymotrypsin, and cyanogen bromide.
The reagent cyanogen bromide cleaves the protein peptides at their internal methionine residues.
The proteolytic enzyme chymotrypsin cleaves the protein peptides at the sites where the
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Biochemistry
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- BiochemistryBiochemistryISBN:9781305961135Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougalPublisher:Cengage Learning