The separation of β -amylase from bovine serum albumin using Sephadex G- 75 as column material is to be discussed. Concept introduction: Sephadex is the trade name for the carbohydrate polymer , Dextran. β -amylase is a globular protein which has a molecular weight of 200 , 000 , whereas the molecular weight of bovine serum albumin is 60 , 000 . In chromatographic techniques, the column generally contains gel particles in the form of porous beads that help in the separation of molecules on the basis of their molecular weight or size. The exclusion limit of the porous gel particles or beads is defined as the size or molecular weight (MW) beyond which molecules often elute in the same void volume of the column as they cannot enter the beads due to their relatively large size.

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Answer 1

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Chapter 5, Problem 20RE

Interpretation Introduction

Interpretation:

The separation of β-amylase from bovine serum albumin using Sephadex G-75 as column material is to be discussed.

Concept introduction:

Sephadex is the trade name for the carbohydrate polymer, Dextran. β-amylase is a globular protein which has a molecular weight of 200,000, whereas the molecular weight of bovine serum albumin is 60,000.

In chromatographic techniques, the column generally contains gel particles in the form of porous beads that help in the separation of molecules on the basis of their molecular weight or size. The exclusion limit of the porous gel particles or beads is defined as the size or molecular weight (MW) beyond which molecules often elute in the same void volume of the column as they cannot enter the beads due to their relatively large size.

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Answer 2

Question

Chapter 5, Problem 20RE

Interpretation Introduction

Interpretation:

The separation of β-amylase from bovine serum albumin using Sephadex G-75 as column material is to be discussed.

Concept introduction:

Sephadex is the trade name for the carbohydrate polymer, Dextran. β-amylase is a globular protein which has a molecular weight of 200,000, whereas the molecular weight of bovine serum albumin is 60,000.

In chromatographic techniques, the column generally contains gel particles in the form of porous beads that help in the separation of molecules on the basis of their molecular weight or size. The exclusion limit of the porous gel particles or beads is defined as the size or molecular weight (MW) beyond which molecules often elute in the same void volume of the column as they cannot enter the beads due to their relatively large size.

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Answer 3

Question

Chapter 5, Problem 20RE

Interpretation Introduction

Interpretation:

The separation of β-amylase from bovine serum albumin using Sephadex G-75 as column material is to be discussed.

Concept introduction:

Sephadex is the trade name for the carbohydrate polymer, Dextran. β-amylase is a globular protein which has a molecular weight of 200,000, whereas the molecular weight of bovine serum albumin is 60,000.

In chromatographic techniques, the column generally contains gel particles in the form of porous beads that help in the separation of molecules on the basis of their molecular weight or size. The exclusion limit of the porous gel particles or beads is defined as the size or molecular weight (MW) beyond which molecules often elute in the same void volume of the column as they cannot enter the beads due to their relatively large size.

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Answer 4

Question

Chapter 5, Problem 20RE

Interpretation Introduction

Interpretation:

The separation of β-amylase from bovine serum albumin using Sephadex G-75 as column material is to be discussed.

Concept introduction:

Sephadex is the trade name for the carbohydrate polymer, Dextran. β-amylase is a globular protein which has a molecular weight of 200,000, whereas the molecular weight of bovine serum albumin is 60,000.

In chromatographic techniques, the column generally contains gel particles in the form of porous beads that help in the separation of molecules on the basis of their molecular weight or size. The exclusion limit of the porous gel particles or beads is defined as the size or molecular weight (MW) beyond which molecules often elute in the same void volume of the column as they cannot enter the beads due to their relatively large size.

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Answer 5

Chapter 5, Problem 20RE

Interpretation Introduction

Interpretation:

The separation of β-amylase from bovine serum albumin using Sephadex G-75 as column material is to be discussed.

Concept introduction:

Sephadex is the trade name for the carbohydrate polymer, Dextran. β-amylase is a globular protein which has a molecular weight of 200,000, whereas the molecular weight of bovine serum albumin is 60,000.

In chromatographic techniques, the column generally contains gel particles in the form of porous beads that help in the separation of molecules on the basis of their molecular weight or size. The exclusion limit of the porous gel particles or beads is defined as the size or molecular weight (MW) beyond which molecules often elute in the same void volume of the column as they cannot enter the beads due to their relatively large size.

Answer 6

Chapter 5, Problem 20RE

Interpretation Introduction

Interpretation:

The separation of β-amylase from bovine serum albumin using Sephadex G-75 as column material is to be discussed.

Concept introduction:

Sephadex is the trade name for the carbohydrate polymer, Dextran. β-amylase is a globular protein which has a molecular weight of 200,000, whereas the molecular weight of bovine serum albumin is 60,000.

In chromatographic techniques, the column generally contains gel particles in the form of porous beads that help in the separation of molecules on the basis of their molecular weight or size. The exclusion limit of the porous gel particles or beads is defined as the size or molecular weight (MW) beyond which molecules often elute in the same void volume of the column as they cannot enter the beads due to their relatively large size.

Answer 7

Chapter 5, Problem 20RE

Interpretation Introduction

Interpretation:

The separation of β-amylase from bovine serum albumin using Sephadex G-75 as column material is to be discussed.

Concept introduction:

Sephadex is the trade name for the carbohydrate polymer, Dextran. β-amylase is a globular protein which has a molecular weight of 200,000, whereas the molecular weight of bovine serum albumin is 60,000.

In chromatographic techniques, the column generally contains gel particles in the form of porous beads that help in the separation of molecules on the basis of their molecular weight or size. The exclusion limit of the porous gel particles or beads is defined as the size or molecular weight (MW) beyond which molecules often elute in the same void volume of the column as they cannot enter the beads due to their relatively large size.

Answer 8

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Answer 10

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Answer 11

Ch. 5 - RECALL What types of homogenization techniques are...Ch. 5 - RECALL When would you choose to use a...Ch. 5 - RECALL What is meant by salting out? How does it...Ch. 5 - RECALL What differences between proteins are...Ch. 5 - RECALL How could you isolate mitochondria from...Ch. 5 - RECALL Can you separate mitochondria from...Ch. 5 - RECALL Give an example of a scenario in which you...Ch. 5 - Prob. 8RE Ch. 5 - REFLECT AND APPLY You are purifying a protein for...Ch. 5 - Prob. 10RE

Solution Summary: The author explains that Sephadex is the trade name for the carbohydrate polymer, Dextran.

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