CAMPBELL BIOLOGY,VOL.II >CUSTOM<
17th Edition
ISBN: 9781323803677
Author: Urry
Publisher: PEARSON
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Textbook Question
Chapter 42.7, Problem 2CC
How does the Bohr shift help deliver O2 to very active tissues?
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 42 Solutions
CAMPBELL BIOLOGY,VOL.II >CUSTOM<
Ch. 42.1 - Prob. 1CCCh. 42.1 - Three-chambered hearts with incomplete septa were...Ch. 42.1 - Prob. 3CCCh. 42.2 - Explain why blood has a higher 02 concentration in...Ch. 42.2 - Prob. 2CCCh. 42.2 - Prob. 3CCCh. 42.3 - Prob. 1CCCh. 42.3 - Prob. 2CCCh. 42.3 - Prob. 3CCCh. 42.4 - Explain why a physician might order a white cell...
Ch. 42.4 - Prob. 2CCCh. 42.4 - Prob. 3CCCh. 42.4 - Prob. 4CCCh. 42.5 - Why is an internal location for gas exchange...Ch. 42.5 - Prob. 2CCCh. 42.5 - MAKE CONNECTIONS Describe similarities in the...Ch. 42.6 - Prob. 1CCCh. 42.6 - Prob. 2CCCh. 42.6 - Prob. 3CCCh. 42.7 - What determines whether O2 and CO2 undergo net...Ch. 42.7 - How does the Bohr shift help deliver O2 to very...Ch. 42.7 - Prob. 3CCCh. 42 - How does the flow of a fluid in a closed...Ch. 42 - Prob. 42.2CRCh. 42 - Prob. 42.3CRCh. 42 - Prob. 42.4CRCh. 42 - Prob. 42.5CRCh. 42 - How does air in the lungs differ from the fresh...Ch. 42 - How are the roles of a respiratory pigment and an...Ch. 42 - Prob. 1TYUCh. 42 - Blood returning to the mammalian heart in a...Ch. 42 - Prob. 3TYUCh. 42 - When you hold your breath, which of the following...Ch. 42 - Prob. 5TYUCh. 42 - Prob. 6TYUCh. 42 - Prob. 7TYUCh. 42 - DRAW IT Plot blood pressure against time for one...Ch. 42 - EVOLUTION CONNECTION One opponent of the movie...Ch. 42 - Prob. 10TYUCh. 42 - Prob. 11TYUCh. 42 - WRITE ABOUT A THEME: INTERACTIONS Some athletes...Ch. 42 - Prob. 13TYU
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