Biology: Life on Earth with Physiology (11th Edition)
Biology: Life on Earth with Physiology (11th Edition)
11th Edition
ISBN: 9780133923001
Author: Gerald Audesirk, Teresa Audesirk, Bruce E. Byers
Publisher: PEARSON
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Chapter 32, Problem 2AC
Summary Introduction

To determine:

Why third degree burns are usually painless. Skin develops from edges of these wounds. Second degree wounds are often very painful. Skin develops from the cells located at the burn edges, in hair follicles and in sweat glands and first degree burns are very painful but heal rapidly from undamaged epidermal cells. Using this concept draw the damage of first-, second- and third-degree burns.

Introduction:

Burns are described as painful wounds caused by thermal, chemical or electrical energy.

Thermal burns are caused by a heat source that raises the temperature if the skin leading to its death and charring. These sources include hot metals, steams, flames and hot liquids. Depending on the severity of the burn these are classified into three categories: first-degree, second-degree, and third-degree burns.

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Overview of Transformation Protocol   -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes.   Questions: 1) What differences would you expect to see between the…
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