Biology: Life on Earth with Physiology (11th Edition)
11th Edition
ISBN: 9780133923001
Author: Gerald Audesirk, Teresa Audesirk, Bruce E. Byers
Publisher: PEARSON
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Chapter 32.1, Problem 1HYEW
Summary Introduction
To determine:
Can you drink too much water?
Introduction:
All the systems in body are dependent on water to work efficiently, such as
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Students have asked these similar questions
1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 32 Solutions
Biology: Life on Earth with Physiology (11th Edition)
Ch. 32.1 - Prob. 1CSCCh. 32.1 - define homeostasis and explain why organisms...Ch. 32.1 - Prob. 1HYEWCh. 32.1 - What would happen if a cold, shvering mammal...Ch. 32.1 - Prob. 2CSCCh. 32.1 - explain the difference between ectotherms and...Ch. 32.1 - Prob. 3CYLCh. 32.1 - Prob. 4CYLCh. 32.2 - Prob. 1CSRCh. 32.2 - By pumping carbon dioxide and other greenhouse...
Ch. 32.2 - Prob. 1CYLCh. 32.2 - Prob. 1ETCh. 32.2 - describe the four types of tissues?Ch. 32.2 - name and describe the major human organ systems?Ch. 32 - Why does life on land present particular...Ch. 32 - The ability of the body to maintain its internal...Ch. 32 - Which of the following statements is False? a....Ch. 32 - Define homeostasis, and explain how negative...Ch. 32 - Prob. 2ACCh. 32 - Prob. 2FIBCh. 32 - Prob. 2MCCh. 32 - Define and compare ectotherms and endotherms....Ch. 32 - Imagine you are a health care professional...Ch. 32 - Prob. 3FIBCh. 32 - Prob. 3MCCh. 32 - Prob. 3RQCh. 32 - Glands with ducts connecting them to the...Ch. 32 - Prob. 4MCCh. 32 - Prob. 4RQCh. 32 - Prob. 5FIBCh. 32 - Which of the following statements is True? a....Ch. 32 - Prob. 5RQCh. 32 - what property distinguishes connective tissue from...Ch. 32 - Prob. 7RQ
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