Microbiology: Principles and Explorations
Microbiology: Principles and Explorations
9th Edition
ISBN: 9781118743164
Author: Jacquelyn G. Black, Laura J. Black
Publisher: WILEY
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Chapter 3, Problem 9SQ

Which of the following stains is used frequently to identify Mycobacterium and other bacteria whose cell walls contain high amounts of lipids?

  1. (a) Gram stain
  2. (b) Schaeffer-Fulton stain
  3. (c) Acid-fast stain
  4. (d) Lipidialar stain
  5. (e) Spore stain
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In lab, we used the Gram stain to differentiate between Gram-positive and Gram-negative bacteria. Some bacteria stain purple (retaining the crystal violet) while others stain pink (from the counter-stain safranin). a) What is the biological molecule that is present in these bacteria that produces different results between Gram-positive and Gram-negative bacteria (be specific!) AND b) What is the difference between Gram-positive and Gram-negative bacteria that creates the different staining patterns (purple vs. pink)? (2-3 sentences max)
After completion of a Gram stain procedure, you notice the bacteria cells shown in the picture below. How would you categorize the bacteria based on 1. Gram stain results, 2. Cell arrangement, and 3. cell shape.? Would the bacteria in question 7 have a thick layer of peptidoglycan or a thin layer of peptidoglycan in its cell wall? Would the bacteria in question 7 have an outer lipopolysaccharide layer in its cell wall? Would the antibiotic penicillin likely work to eliminate infection with the bacteria in question 7? How would you describe the type of flagella arrangement for the bacterium below? (You'll use one word for the description here.)
In the space provided below, give me the word(s) that would correctly complete each sentence: A) The equation "d = A/ (2 NA)" describes the of a lens system B) In the flagella stain, the mordant makes bacterial flagella easier to see by making them
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