Microbiology: Principles and Explorations
Microbiology: Principles and Explorations
9th Edition
ISBN: 9781118743164
Author: Jacquelyn G. Black, Laura J. Black
Publisher: WILEY
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Chapter 3, Problem 2CTQ
Summary Introduction

To describe: The observation that Person C saw when he observed his completed slide under oil immersion and explain the reason.

Introduction: Gram staining is also called Gram stain or Gram’s method of staining, which is used to classify and distinguish bacterial species into two large groups. It includes Gram-negative and Gram-positive. Gram staining differentiates bacteria by the physical and chemical properties of their cell walls.

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John performed a side-by-side Gram satin of a known Gram positive and known Gram negative organism. Unfortunately, he forgot to do iodine step. When he observed his completed slide under oil immersion, what do you think he saw?
Since the dyes for the Gram stain are not specific to the bacteria theystain, what mistakes in the procedure could cause an incorrect result?
Jessica placed crystal violet ,grama's iodine and safranin reagents on her bench.Which staining procedure is she preparing for with these reagents? a)Grama's staining procedure b)simple staining procedure c)Acid -fast staining procedure d)Non -acid fast staining procedure e)Scaeffer -Fulton staining procedure
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