Microbiology: Principles and Explorations
9th Edition
ISBN: 9781118743164
Author: Jacquelyn G. Black, Laura J. Black
Publisher: WILEY
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Chapter 3, Problem 2CTQ
Summary Introduction
To describe: The observation that Person C saw when he observed his completed slide under oil immersion and explain the reason.
Introduction: Gram staining is also called Gram stain or
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John performed a side-by-side Gram satin of a known Gram positive and known Gram negative organism. Unfortunately, he forgot to do iodine step. When he observed his completed slide under oil immersion, what do you think he saw?
Since the dyes for the Gram stain are not specific to the bacteria theystain, what mistakes in the procedure could cause an incorrect result?
Jessica placed crystal violet ,grama's iodine and safranin reagents on her bench.Which staining procedure is she preparing for with these reagents?
a)Grama's staining procedure
b)simple staining procedure
c)Acid -fast staining procedure
d)Non -acid fast staining procedure
e)Scaeffer -Fulton staining procedure
Chapter 3 Solutions
Microbiology: Principles and Explorations
Ch. 3 - Prob. 1.1SCCh. 3 - Prob. 1.2SCCh. 3 - Why would radio waves and microwaves be unsuitable...Ch. 3 - Would standard immersion oil placed on a plastic...Ch. 3 - Prob. 2.1SCCh. 3 - How can you distinguish between TEM and SEM...Ch. 3 - Prob. 2.3SCCh. 3 - Prob. 2.4SCCh. 3 - What can be observed in a wet-mount or...Ch. 3 - What is the difference between simple stains and...
Ch. 3 - What color are Gram-negative organisms after Gram...Ch. 3 - Prob. 1CCSCh. 3 - Prob. 1CTQCh. 3 - Prob. 2CTQCh. 3 - What are the advantages and disadvantages of...Ch. 3 - Prob. 1SQCh. 3 - Prob. 2SQCh. 3 - Prob. 3SQCh. 3 - Prob. 4SQCh. 3 - Match the following:Ch. 3 - Prob. 6SQCh. 3 - Prob. 7SQCh. 3 - Prob. 8SQCh. 3 - Which of the following stains is used frequently...Ch. 3 - Prob. 10SQCh. 3 - Prob. 11SQCh. 3 - Which of the following can give you ambiguous...Ch. 3 - Prob. 13SQCh. 3 - Prob. 14SQCh. 3 - Prob. 15SQCh. 3 - Prob. 16SQCh. 3 - Which of the following microscopic techniques...Ch. 3 - Prob. 18SQCh. 3 - Prob. 19SQCh. 3 - Prob. 20SQCh. 3 - Prob. 21SQ
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- After performing the gram stain you observe your result as gram negative. To help confirm this result what type of method (besides the gram stain) could you perform? Please explain why, the result you would want to observe and the interpretation of the result.arrow_forwardWhat is the function of the mordant used in the Gram staining procedure? If the iodine step is skipped, what color are Gram-negative cells observedarrow_forwardWhy is oil immersion necessary to see bacteria in the laboratory?arrow_forward
- After performing the gram stain you observe your result as gram negative. To help confirm this result what type of method (besides the gram stain) could you perform? Please explainwhy, the result you would want to observe and the interpretation of the result.arrow_forwardif an unknown bacterial slide appears to be in fading color and the shape of the specimen seems to be diffused. what troubleshooting of the focusing is needed in order to see the bacteria clearly?arrow_forwardwhy are the steps in gram staining are so carefully standardized?arrow_forward
- The bacterial cells in the image below were stained with a high concentration of carbolfuschin for an extended period of time (5 minutes) while the slide was heated. The slide was decolorized with a mixture of acid and alcohol. The slide was then counter-stained with methylene blue. What kind of stain does this describe?arrow_forwardWhy is Iodine used in the Gram Staining procedure?arrow_forwardWhy is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?arrow_forward
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