Microbiology: Principles and Explorations
Microbiology: Principles and Explorations
9th Edition
ISBN: 9781118743164
Author: Jacquelyn G. Black, Laura J. Black
Publisher: WILEY
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Chapter 3, Problem 1CCS
Summary Introduction

To describe: The ways by which a technician can determine whether the bacterium he cultured from the patient’s specimen is one of the three suspected organisms.

Introduction: Bacteria are microscopic, single-celled organisms that thrive in different environments. These organisms can survive in soil, ocean, and inside the gut of a human. Bacterial diseases include any type of illness which is caused by bacteria.

Summary Introduction

To describe: The kind of microscope that the technician should use to examine each slide.

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Two patients, with identical medical histories are in adjacent hospital beds. Each is infected with Streptococcus pneumoniae. Patient A's infection progresses to pneumonia while Patient B's infection resolves quickly. The microbial stain of the lung fluid from Patient A indicated the bacterial cells had a capsule, while Patient B's lung fluid stain did not. Why did the capsule affect the course of the infection in Patient A?
The following pictures show the results of a Disk Diffusion Assay for different types of bacteria. For each bacteria, what antibiotic would you recommend be used on the patient? Explain your choice.
Note that it is not appropriate to self-diagnose outside of a medical context and this is a completely hypothetical scenario. Imagine you have a rash on your foot. You're concerned that it's an infection and inoculate a sample onto an agar plate. You wonder, How can I figure out whether the pathogen is a bacterium vs a eukaryote? You decide to use lab supplies to get a basic understanding of the pathogen. Be specific about what tests you use and what you expect the results to be. Limit yourself to experiments we could do in our lab. What is one experiment you could do, involving culturing the organism?
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