Microbiology: Principles and Explorations
9th Edition
ISBN: 9781118743164
Author: Jacquelyn G. Black, Laura J. Black
Publisher: WILEY
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Chapter 3, Problem 6SQ
Summary Introduction
Introduction: The microscope is an optical instrument used for viewing very small objects, even cells. It commonly used for scientific and medical purposes. Simple microscope, compound microscope, optical microscope, scanning electron microscope, and transmission electron microscope are the different types of microscopes.
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The total magnification of a microscope is calculated by:(a) Addition of the objective lens and ocular lens magnifi-cation powers(b) Multiplication of the objective lens and ocular lens mag-nification powers(c) Multiplication of the objective lens and condenser lensmagnification powers(d) The objective lens power squared(e) None of the above
Define each of the following terms:
A) What is resolution and how is resolution related to the wavelength of light used to illuminate the sample?
B) What is the magnification of the specimen if you are using a 40x objective and a 10x eyepiece?
C) How is the numerical aperture (NA) of a lens related to its ability to gather light from a specimen?
What is the final magnification that you would obtain if you were viewing an object through a microscope with a 40X objective lens and a 25.5X ocular lens?
Chapter 3 Solutions
Microbiology: Principles and Explorations
Ch. 3 - Prob. 1.1SCCh. 3 - Prob. 1.2SCCh. 3 - Why would radio waves and microwaves be unsuitable...Ch. 3 - Would standard immersion oil placed on a plastic...Ch. 3 - Prob. 2.1SCCh. 3 - How can you distinguish between TEM and SEM...Ch. 3 - Prob. 2.3SCCh. 3 - Prob. 2.4SCCh. 3 - What can be observed in a wet-mount or...Ch. 3 - What is the difference between simple stains and...
Ch. 3 - What color are Gram-negative organisms after Gram...Ch. 3 - Prob. 1CCSCh. 3 - Prob. 1CTQCh. 3 - Prob. 2CTQCh. 3 - What are the advantages and disadvantages of...Ch. 3 - Prob. 1SQCh. 3 - Prob. 2SQCh. 3 - Prob. 3SQCh. 3 - Prob. 4SQCh. 3 - Match the following:Ch. 3 - Prob. 6SQCh. 3 - Prob. 7SQCh. 3 - Prob. 8SQCh. 3 - Which of the following stains is used frequently...Ch. 3 - Prob. 10SQCh. 3 - Prob. 11SQCh. 3 - Which of the following can give you ambiguous...Ch. 3 - Prob. 13SQCh. 3 - Prob. 14SQCh. 3 - Prob. 15SQCh. 3 - Prob. 16SQCh. 3 - Which of the following microscopic techniques...Ch. 3 - Prob. 18SQCh. 3 - Prob. 19SQCh. 3 - Prob. 20SQCh. 3 - Prob. 21SQ
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- If the ocular lens of a microscope has 10X magnifying power, and the 40X objective lens is being used for observation, what is the total magnification for the viewed image? 10x 43x 1000x 400xarrow_forwardHow will the following affect resolution during microscopy? I) Closing or opening the diaphragm II) Raising or lowering the condenser III) Increasing or reducing the light intensityarrow_forwardPlease explain the tablearrow_forward
- Differentiate between the limit of resolution of the typical light microscope and that of the unaided human eyes the relationship between the depth of field and magnification is that When magnification increases, the depth of field decreases. Name two procedures (steps) you should do to achieve the maximum resolution when using the oil immersion lens State the relationship between the working distance of an objective lens and its magnification power “The coarse focus knob can be used to adjust the focus when using any of the objective lenses”. Is this statement true or false? If it is false, please correct it. When using the oil immersion lens but you cannot focus, what should you should not do? And what you should do? What does this statement mean: “Your microscope is parfocal”? What is the significance of condenser lens? Multiple choice question: The most useful adjustment for increasing image contrast in low power magnification is _______ Closing down the diaphragm Closing one…arrow_forwardComplete the following table by calculating the total magnification of the different objectives of the microscope with the given eyepieces. OBJECTIVES OCULAR Designation Magnification Focal Length Numerical Aperture (NA) Final magnification with: 10x 15x 25x Scanner 4x 46mm 0.10 LPO 10x 16mm 0.25 HPO 40x 4mm 0.55 OIO 100x 1.8mm 1.30arrow_forwarda)What is the condenser lens on a compound microscope, and how can it affect your view of your specimen? b)Calculate the total magnification when looking through the 40X lens on the compound microscope. c) Describe the process to get a specimen into view using the 40X objective starting from the point after you have plugged in your scope and cleaned the objectives and slide.arrow_forward
- Calculate the diameter of the field of view for each total magnification on your microscope in millimeters (mm) and then convert this value to micrometers (um): 4.5 mm = 4,500 um Scanning (40X): 1.8 mm x 100X/40X = Low power (100X): FOV diameter 1.8 mm = 1,800 um High power (400X): 1.8 mm x 100X/400X = 0.45 mm = 450 um Oil immersion (1000X): O.18m 180 um 1.8 mm x 100x/1000X = Draw and estimate the length of a single Euglena (high power) and Paramecium (low power): Paramecium Euglena total magnification total magnification 400x yoox FOV diam. FOV diam. um uni length length umarrow_forwardThe following questions talk about increasing magnification in microscopes. Please answer questions 1 and 2 After a specimen is in focus, where in the field of view should position the specimen before increasing the magnification (be precise)? (QUESTION 2 HAS TWO PARTS TO IT): To increase the magnification, which objective should you click into position next? After refocusing the specimen with the new objective, if you want to increase the magnification even more, which objective should you now click into position?arrow_forwardFor a microscope that has an objective lens magnification of 100X and an ocular lens magnification of 5X, calculate the total magnification. 500X O 1000X O 50X O 100X O No listed answer is correct.arrow_forward
- If the total magnification of a microscope is 700X and the ocular lens magnifies by a factor of 10x, what is the magnification of the objective lens that was used? Paragraph BIUarrow_forwardUsing a good compound light microscope with a resolving of 0.3 micro meter a 10x ocular lens ,and a 100 x oil immersion lens, would you be able to discern two objects separated by 0.4 micro meter?0.2 micro meter? 300 neno meter ? Explain your reasoning.arrow_forwardPrevious question: For example, you can estimate the field of view diameter at 400x total magnification using the measured diameter at 100x total magnification. The magnification is 4 times greater so the field of view will be 4 times smaller. What is the field of view under the 40X objective of the microscope. - The Answer I got for this question was 3/4mm.arrow_forward
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