Microbiology: Principles and Explorations
9th Edition
ISBN: 9781118743164
Author: Jacquelyn G. Black, Laura J. Black
Publisher: WILEY
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
Chapter 3, Problem 16SQ
Summary Introduction
Introduction: Staining is a supplementary method that provides divergence to the microscopic image for better vision under the microscope. This technique is widely used for the examination of tissues, cells, and cellular components.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
The presence of a capsule around bacterial cells usually indi-cates their increased disease-causing potential and resistance todisinfection. Capsules are generally viewed by:(a) Spore staining(b) Scanning electron microscopy(c) Gram staining(d) Ziehl-Neelsen staining(e) Negative staining
Differentiate the staining procedures.
Gram Stain
Acid Fast
Endospore
Capsule
Flagella
a) Principle
b) Characteristic of
Bacteria viewed using
this stain. (e.g.
bacterial type, strain,
or targeting particular
bacterial feature/s)
c) Stain/s Used
d) Type of Stain
e) Mechanism of Staining
(of each stain used)
f) Flow of Method
In lab, we used the Gram stain to differentiate between Gram-positive and Gram-negative
bacteria. Some bacteria stain purple (retaining the crystal violet) while others stain pink (from the
counter-stain safranin).
a) What is the biological molecule that is present in these bacteria that produces different results
between Gram-positive and Gram-negative bacteria (be specific!) AND
b) What is the difference between Gram-positive and Gram-negative bacteria that creates the
different staining patterns (purple vs. pink)?
(2-3 sentences max)
Chapter 3 Solutions
Microbiology: Principles and Explorations
Ch. 3 - Prob. 1.1SCCh. 3 - Prob. 1.2SCCh. 3 - Why would radio waves and microwaves be unsuitable...Ch. 3 - Would standard immersion oil placed on a plastic...Ch. 3 - Prob. 2.1SCCh. 3 - How can you distinguish between TEM and SEM...Ch. 3 - Prob. 2.3SCCh. 3 - Prob. 2.4SCCh. 3 - What can be observed in a wet-mount or...Ch. 3 - What is the difference between simple stains and...
Ch. 3 - What color are Gram-negative organisms after Gram...Ch. 3 - Prob. 1CCSCh. 3 - Prob. 1CTQCh. 3 - Prob. 2CTQCh. 3 - What are the advantages and disadvantages of...Ch. 3 - Prob. 1SQCh. 3 - Prob. 2SQCh. 3 - Prob. 3SQCh. 3 - Prob. 4SQCh. 3 - Match the following:Ch. 3 - Prob. 6SQCh. 3 - Prob. 7SQCh. 3 - Prob. 8SQCh. 3 - Which of the following stains is used frequently...Ch. 3 - Prob. 10SQCh. 3 - Prob. 11SQCh. 3 - Which of the following can give you ambiguous...Ch. 3 - Prob. 13SQCh. 3 - Prob. 14SQCh. 3 - Prob. 15SQCh. 3 - Prob. 16SQCh. 3 - Which of the following microscopic techniques...Ch. 3 - Prob. 18SQCh. 3 - Prob. 19SQCh. 3 - Prob. 20SQCh. 3 - Prob. 21SQ
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Examine the image below. The bacteria in this image have been treated with gram straining procedure. Indicate the following information A. Shape ........; Gram stain ( Gram (+), Gram (-) or both ... B. Stained color under microscope..... . C. Is the PG wall think, thin or both ..arrow_forwardIn the space provided below, give me the word(s) that would correctly complete each sentence: A) The equation "d = A/ (2 NA)" describes the of a lens system B) In the flagella stain, the mordant makes bacterial flagella easier to see by making themarrow_forwarda. What can you observe in viewing your stained bacterial slide under the microscope if you fixed a lot of bacterial colonies in your slide during smear preparation? b. What can you observe in viewing your stained bacterial slide under the microscope if you heat fixed your slide in a much longer exposure to heat?arrow_forward
- All except which of the following are important aspects to consider in determining the G+C content of a microorganism? a) determination of gram stain b)double stranded dna absorbs less light than single DNA strang c)he greater the G+C content, the higher the temperature required to denature DNA d) 3 hydrogren bonds between G+C content vs 2 hydrogen bonds between A and Tarrow_forward4) In Gram Stain the bacteria that becomes colorless after washing with alcohol or acetone is the: a) Gram +ve bacteria b) Gram -ve bacteria c) ) Gram neutral bacteria d) archaebacteria e) b & c5) Antimetabolites include: a) Sulfonamides b) Tetracyclins c) Trimethoprim d) all of the above e) a and c6) Oil immersion is used in light microscope because: a) it increases only b) it decreases only c) it increases the / n sin value d) it decreases / n sin e) same refractive index as the glass slidearrow_forwardThis part of the staining process helps the primary stain to remain in the cell during decolorization. O 1) Secondary stain O 2) primary stain O 3) counterstain O 4) fixing reagent O 5) mordantarrow_forward
- The resolution of a microscope is affected by the wavelength of light used to examine the sample. O 1) True O 2) Falsearrow_forwardAfter completion of a Gram stain procedure, you notice the bacteria cells shown in the picture below. How would you categorize the bacteria based on 1. Gram stain results, 2. Cell arrangement, and 3. cell shape.? Would the bacteria in question 7 have a thick layer of peptidoglycan or a thin layer of peptidoglycan in its cell wall? Would the bacteria in question 7 have an outer lipopolysaccharide layer in its cell wall? Would the antibiotic penicillin likely work to eliminate infection with the bacteria in question 7? How would you describe the type of flagella arrangement for the bacterium below? (You'll use one word for the description here.)arrow_forwardThe diffraction limit describes the limitationof light microscopes to distinguish objects less than 200 microns apart. Most viruses are less than 200 microns in size. (a) Brefly describe one molecular technique and one microscopy technique that could help you identify the specific virus infecting your patient. Be sure to wxplain how the technique works to identify virus particles. (b) Make an argument for which of the techniques would be best to use during clinical diagnosis.arrow_forward
- if a bacteria under observation in a microscope appears to be blurry, what measures are needed to do in the microscope in order to see and identify it clearly?arrow_forwardAnswer the following questions What is the purpose of the 95% ethanol step in the Gram staining procedure? (1 Mark) xiii) What is the function of the following reagents used in Gram stain: a) Crystal Violet b) Gram’s iodine c) Acetone/alcohol d) Safranin Iarrow_forwardIdentify the texture, transparency, color, colony size, form, margin and elevation of the two colonies in the photo (a) and (b)arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education