Microbiology: Principles and Explorations
9th Edition
ISBN: 9781118743164
Author: Jacquelyn G. Black, Laura J. Black
Publisher: WILEY
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Chapter 3, Problem 19SQ
Summary Introduction
Introduction: A microscope is an optical instrument used for viewing very small objects, even cells. It is commonly used for scientific and medical purposes. Simple microscope, compound microscope, optical microscope, scanning electron microscope, and transmission electron microscope are the different types of microscopes.
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Place the path of light (using the numbers) in the microscope in the proper order as the light
travels from its source until it hits the researcher’s eye: 1) condenser 2) light source 3) nose-
piece lens 4) ocular lens 5) slide
a) 1, 2, 5, 4, 3 b) 1, 3, 4, 2, 5 c) 2, 1, 5, 3, 4 d) 3, 2, 1, 4, 5 e) 1, 3, 4, 2, 5
If a microscope has a 40x objective lens in place and a 15x ocular lens, the total magnification of the microscope system under these conditions is _____.
a)
600x
b)
2.7x
c)
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d)
30x
If you were trying to visualize living organisms without staining, which type of
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O 1) brightfield
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Chapter 3 Solutions
Microbiology: Principles and Explorations
Ch. 3 - Prob. 1.1SCCh. 3 - Prob. 1.2SCCh. 3 - Why would radio waves and microwaves be unsuitable...Ch. 3 - Would standard immersion oil placed on a plastic...Ch. 3 - Prob. 2.1SCCh. 3 - How can you distinguish between TEM and SEM...Ch. 3 - Prob. 2.3SCCh. 3 - Prob. 2.4SCCh. 3 - What can be observed in a wet-mount or...Ch. 3 - What is the difference between simple stains and...
Ch. 3 - What color are Gram-negative organisms after Gram...Ch. 3 - Prob. 1CCSCh. 3 - Prob. 1CTQCh. 3 - Prob. 2CTQCh. 3 - What are the advantages and disadvantages of...Ch. 3 - Prob. 1SQCh. 3 - Prob. 2SQCh. 3 - Prob. 3SQCh. 3 - Prob. 4SQCh. 3 - Match the following:Ch. 3 - Prob. 6SQCh. 3 - Prob. 7SQCh. 3 - Prob. 8SQCh. 3 - Which of the following stains is used frequently...Ch. 3 - Prob. 10SQCh. 3 - Prob. 11SQCh. 3 - Which of the following can give you ambiguous...Ch. 3 - Prob. 13SQCh. 3 - Prob. 14SQCh. 3 - Prob. 15SQCh. 3 - Prob. 16SQCh. 3 - Which of the following microscopic techniques...Ch. 3 - Prob. 18SQCh. 3 - Prob. 19SQCh. 3 - Prob. 20SQCh. 3 - Prob. 21SQ
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- What advantage do fluorescent dyes and fluorescence microscopy provide in comparison to the chemical dyes used to stain specimens for light microscopy?arrow_forwardHow many types of objective lenses a light microscope has?arrow_forwardHow will the following affect resolution during microscopy? I) Closing or opening the diaphragm II) Raising or lowering the condenser III) Increasing or reducing the light intensityarrow_forward
- What’s the aim of using a microscope for examining? What can the kohler illumination technique tell us? What are the sources of error that can impact the data ?arrow_forward2. The boy is wondering what to use to clean the microscope lenses. The lens tissue has been treated to prevent scratching. What should one use to clean? Why? 3. The girl is focusing a slide and she is moving the stage up toward the slide. a) What has happened to the slide? b) s this the proper way to focus? c) Should you focus by moving stage up or down? d) What happens to a microscope i place at the edge? e) What should you do to prevent this from happening. Fgutearrow_forwarda. Do you open both eyes or close one eye when viewing a specimen under a microscope? Why? Why not? b. In using a compound microscope, do you start with the low power objective (LPO) or the high power objective (HPO)? Why or Why not? c. When using 60X, do you it together with the coarse adjustment knob or fine adjustment knob? d. Why must you slowly turn the fine adjustment knob? e. Is natural light or is artificial light used when using the microscope?arrow_forward
- a)What is the condenser lens on a compound microscope, and how can it affect your view of your specimen? b)Calculate the total magnification when looking through the 40X lens on the compound microscope. c) Describe the process to get a specimen into view using the 40X objective starting from the point after you have plugged in your scope and cleaned the objectives and slide.arrow_forwardWhat are the main characteristics of scanning electron microscopy?arrow_forwardWhat is a wet mount technique in microscopy?arrow_forward
- Rowena was tasked to measure the actual magnification of her specimen mounted on a slide without drawing or taking a photomicrograph. Which type of microscope should she use? Why? How does it help her get the actual magnification?arrow_forward11) What happens to the field of view with respect to magnification? a.) As magnification decreases, the field of view increases. b.) As magnification increases, the field of view decreases c.) The relationship between magnification and the field of view remains the samearrow_forward3D dimensionality is a limitation of the compound microscope. Depth of field, DOF, describes dimensionality form top to bottom and can be observed with colored cross threads. Observe the crossed thread slide on low power (4x), then on medium power (10x), then on high power (40x objective magnification). Which crossed fiber is on top? How do you know?arrow_forward
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