Microbiology: Principles and Explorations
9th Edition
ISBN: 9781118743164
Author: Jacquelyn G. Black, Laura J. Black
Publisher: WILEY
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Textbook Question
Chapter 3, Problem 17SQ
Which of the following microscopic techniques provide three-dimensional images of a bacterial cell?
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You perform a ten-fold serial dilution of a bacterial culture to determine the number of colony
forming units (CFU) per mL in the culture. You then do a plate count with these growth results
(no. of colonies): 1:10: too many to count; 1:100 too many to count; 1:1,000: 126 colonies;
1:10,000 14 colonies; 1:100,000: no growth
The number of CFU per mL in the original culture was:
1 ml
1 ml
1 ml
1 ml
Original
inoculum
Dilutions
1,000
126
9 ml broth
in each tube
126,000
10,000
140,000
1:10
1 ml
1:100
1 ml
1:1000
1 ml
1 ml
1:10,000
1 ml
1:100,000
1 ml
What is the importance of describing the morphological characteristics of bacterial colonies when cultures were sampled from an environmental source? Explain and justify your points.
How can colony morphology be used in the identification of bacterial species?
What are the environmental factors that affect the growth of bacteria?
What is agar (or agar-agar)? Is this different from nutrient agar? Why?
How is xylol used to clean the lens of a microscope? What may happen if xylol is constantly used for this purpose?
For what purpose would you adjust each of the following microscope components during a microscopy exercise?
iris diaphragm
coarse adjustment knob
fine adjustment knob
condenser
mechanical stage control
Chapter 3 Solutions
Microbiology: Principles and Explorations
Ch. 3 - Prob. 1.1SCCh. 3 - Prob. 1.2SCCh. 3 - Why would radio waves and microwaves be unsuitable...Ch. 3 - Would standard immersion oil placed on a plastic...Ch. 3 - Prob. 2.1SCCh. 3 - How can you distinguish between TEM and SEM...Ch. 3 - Prob. 2.3SCCh. 3 - Prob. 2.4SCCh. 3 - What can be observed in a wet-mount or...Ch. 3 - What is the difference between simple stains and...
Ch. 3 - What color are Gram-negative organisms after Gram...Ch. 3 - Prob. 1CCSCh. 3 - Prob. 1CTQCh. 3 - Prob. 2CTQCh. 3 - What are the advantages and disadvantages of...Ch. 3 - Prob. 1SQCh. 3 - Prob. 2SQCh. 3 - Prob. 3SQCh. 3 - Prob. 4SQCh. 3 - Match the following:Ch. 3 - Prob. 6SQCh. 3 - Prob. 7SQCh. 3 - Prob. 8SQCh. 3 - Which of the following stains is used frequently...Ch. 3 - Prob. 10SQCh. 3 - Prob. 11SQCh. 3 - Which of the following can give you ambiguous...Ch. 3 - Prob. 13SQCh. 3 - Prob. 14SQCh. 3 - Prob. 15SQCh. 3 - Prob. 16SQCh. 3 - Which of the following microscopic techniques...Ch. 3 - Prob. 18SQCh. 3 - Prob. 19SQCh. 3 - Prob. 20SQCh. 3 - Prob. 21SQ
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