Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 3, Problem 9RQ
Summary Introduction
Interpretation:
The osmolarity of
Concept introduction:
The concentration of a solution that contributes to the osmotic pressure, which is expressed in osmoles of solute per liter of solution.
An osmole is defined as 1 mol of particles that contribute toward the osmotic pressure of the solution.
Osmolarity of a solution is obtained by multiplying the molarity with the number of osmoles produced by the solute of the solution.
It is donated by the unit osmole.
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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism
of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help
To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?
To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!
Chapter 3 Solutions
Biochemistry: The Molecular Basis of Life
Ch. 3 - Prob. 1QCh. 3 - Prob. 2QCh. 3 - Prob. 3QCh. 3 - Prob. 4QCh. 3 - Prob. 1RQCh. 3 - Prob. 2RQCh. 3 - Prob. 3RQCh. 3 - Prob. 4RQCh. 3 - Prob. 5RQCh. 3 - Prob. 6RQ
Ch. 3 - Prob. 7RQCh. 3 - Prob. 8RQCh. 3 - Prob. 9RQCh. 3 - Prob. 10RQCh. 3 - Prob. 11RQCh. 3 - Prob. 12RQCh. 3 - Prob. 13RQCh. 3 - Prob. 14RQCh. 3 - Prob. 15RQCh. 3 - Prob. 16RQCh. 3 - Prob. 17RQCh. 3 - Prob. 18RQCh. 3 - Prob. 19RQCh. 3 - Prob. 20RQCh. 3 - Prob. 21RQCh. 3 - Prob. 22RQCh. 3 - Prob. 23RQCh. 3 - Prob. 24RQCh. 3 - Prob. 25RQCh. 3 - Prob. 26RQCh. 3 - Prob. 27RQCh. 3 - Prob. 28RQCh. 3 - Prob. 29RQCh. 3 - Prob. 30RQCh. 3 - Prob. 31RQCh. 3 - Prob. 32RQCh. 3 - Prob. 33RQCh. 3 - Prob. 34RQCh. 3 - Prob. 35RQCh. 3 - Prob. 36FBCh. 3 - Prob. 37FBCh. 3 - Prob. 38FBCh. 3 - Prob. 39FBCh. 3 - Prob. 40FBCh. 3 - Prob. 41FBCh. 3 - Prob. 42FBCh. 3 - Prob. 43FBCh. 3 - Prob. 44FBCh. 3 - Prob. 45FBCh. 3 - Prob. 46SACh. 3 - Prob. 47SACh. 3 - Prob. 48SACh. 3 - Prob. 49SACh. 3 - Prob. 50SACh. 3 - Prob. 51TQCh. 3 - Prob. 52TQCh. 3 - Prob. 53TQCh. 3 - Prob. 54TQCh. 3 - Prob. 55TQCh. 3 - Prob. 56TQCh. 3 - Prob. 57TQCh. 3 - Prob. 58TQCh. 3 - Prob. 59TQCh. 3 - Prob. 60TQCh. 3 - Prob. 61TQCh. 3 - Prob. 62TQCh. 3 - Prob. 63TQCh. 3 - Prob. 64TQCh. 3 - Prob. 65TQCh. 3 - Prob. 66TQCh. 3 - Prob. 67TQCh. 3 - Prob. 68TQCh. 3 - Prob. 69TQCh. 3 - Prob. 70TQCh. 3 - Prob. 71TQCh. 3 - Prob. 72TQCh. 3 - Prob. 73TQCh. 3 - Prob. 74TQCh. 3 - Prob. 75TQCh. 3 - Prob. 76TQ
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