Biochemistry: Concepts and Connections (2nd Edition)
Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Chapter 3, Problem 19P

a. Consider the malate dehydrogenase reaction, which is part of the citric acid cycle:

malate + NAD+ oxaloacetate + NADH + H+

In yeast mitochondria, where the pH= 8.1, this reaction is exergonic only at low oxaloacetate concentrations. Assuming a pH = 8.1, a temperature of 37o C, and the steady-state concentrations given below, calculate the maximum concentration of oxaloacetate at which the reaction will still be exergonic.

Chapter 3, Problem 19P, a. Consider the malate dehydrogenase reaction, which is part of the citric acid cycle: malate + NAD+

b. How would a drop in pH affect Δ G for the reaction described in part (a)?
c. How would an increase in intracellular malate levels affect Δ G for the reaction in part (a)?
d. How would an increase in intracellular pyruvate levels affect Δ G for the reaction in part (a)?

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