The double-displacement (ping-pong) catalytic mechanism needs to be determined for the glutamate oxaloacetate transaminase I. Concept Introduction: A double displacement reaction follows a bimolecular mechanism. In the mechanism, two reactants say XY and MN reacts to form XM and YN product. This is due to the nucleophilic attack of 1 group on another which results in the release of groups to form new compound. The enzymatic double displacement reaction is also known as ping-pong reaction. It takes place when 1 or more products are formed before the binding of all substrate to the enzyme. In the mechanism, the substrate bounces on and off the enzyme like a ping pong ball.

Question

9) Below, there is a representation of an SDS-PAGE gel. Assuming the samples in the MW standard have masses of: 66 kDa, 45 kDa, 36 kDa, 29 kDa, 24 kDa, 20.1 kDa, and 14.2 kDa, a) Figure 4: indicate where each of the measurements were taken and label as in II.6. figure 2 above. b) As in II.7. Table 1 above create Table 2 using the data below. Determine the r.f. values for the MW standards, plot the relative mobility versus the log of the mass for the standards, and use the best fit straight line to determine the molecular weights of the proteins in the whey, peak 1, and peak 2 lanes. (5 pts—this will be scaled up appropriately if your gel did not develop properly) dye MW Whey Peak 1 Peak 2

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Chapter 25, Problem 22P

Interpretation Introduction

Interpretation:

The double-displacement (ping-pong) catalytic mechanism needs to be determined for the glutamate oxaloacetate transaminase I.

Concept Introduction:

A double displacement reaction follows a bimolecular mechanism. In the mechanism, two reactants say XY and MN reacts to form XM and YN product. This is due to the nucleophilic attack of 1 group on another which results in the release of groups to form new compound. The enzymatic double displacement reaction is also known as ping-pong reaction. It takes place when 1 or more products are formed before the binding of all substrate to the enzyme.

In the mechanism, the substrate bounces on and off the enzyme like a ping pong ball.

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