Biochemistry
9th Edition
ISBN: 9781305961135
Author: Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher: Cengage Learning
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Chapter 21, Problem 54RE
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The way in which leptin affects the
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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism
of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help
To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?
To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!
Chapter 21 Solutions
Biochemistry
Ch. 21 - REFLECT AND APPLY (a) The major energy storage...Ch. 21 - RECALL What is the difference between...Ch. 21 - RECALL How are lipases activated hormonally?Ch. 21 - RECALL What is the metabolic purpose of linking a...Ch. 21 - RECALL Outline the role of carnitine in the...Ch. 21 - RECALL What is the difference between the type of...Ch. 21 - RECALL Draw a six-carbon saturated fatty acid and...Ch. 21 - REFLECT AND APPLY Why does the degradation of...Ch. 21 - REFLECT AND APPLY Given the nature of the hormonal...Ch. 21 - RECALL Compare the energy yields from the...
Ch. 21 - RECALL Which generates more ATPthe processing of...Ch. 21 - MATHEMATICAL Calculate the ATP yield for the...Ch. 21 - REFLECT AND APPLY It is frequently said that...Ch. 21 - RECALL Describe briefly how -oxidation of an...Ch. 21 - RECALL You hear a fellow student say that the...Ch. 21 - RECALL What are the unique enzymes needed to...Ch. 21 - RECALL What are the unique enzymes needed to...Ch. 21 - MATHEMATICAL Calculate the net ATP yield from the...Ch. 21 - MATHEMATICAL Calculate the net ATP yield from...Ch. 21 - MATHEMATICAL Calculate the net ATP yield from...Ch. 21 - REFLECT AND APPLY How many cycles of -oxidation...Ch. 21 - REFLECT AND APPLY It has been stated many times...Ch. 21 - RECALL Under what conditions are ketone bodies...Ch. 21 - RECALL Briefly outline the reactions involved in...Ch. 21 - REFLECT AND APPLY Why might a doctor smell the...Ch. 21 - REFLECT AND APPLY Why might a person who is an...Ch. 21 - REFLECT AND APPLY A friend who is trying to lose...Ch. 21 - RECALL Compare and contrast the pathways of fatty...Ch. 21 - RECALL Outline the steps involved in the...Ch. 21 - RECALL What is the metabolic importance of...Ch. 21 - RECALL In fatty acid degradation, we encounter...Ch. 21 - RECALL How are the two redox reactions of...Ch. 21 - RECALL How is ACP similar to coenzyme A? How is it...Ch. 21 - RECALL What is the purpose of having ACP as a...Ch. 21 - RECALL Why are linoleate and linolenate considered...Ch. 21 - REFLECT AND APPLY Is it possible to convert fatty...Ch. 21 - REFLECT AND APPLY What is the role of citrate in...Ch. 21 - REFLECT AND APPLY In the mitochondrion is a...Ch. 21 - REFLECT AND APPLY In fatty acid synthesis,...Ch. 21 - REFLECT AND APPLY (a) Where in an earlier chapter...Ch. 21 - RECALL What is the source of the glycerol in...Ch. 21 - RECALL What is the activating group used in the...Ch. 21 - RECALL What are the differences between synthesis...Ch. 21 - RECALL How are isoprene units important in...Ch. 21 - RECALL A cholesterol sample is prepared using...Ch. 21 - Prob. 46RECh. 21 - Prob. 47RECh. 21 - Prob. 48RECh. 21 - Prob. 49RECh. 21 - REFLECT AND APPLY A drug that reduces blood...Ch. 21 - RECALL What role does neuropeptide Y play in...Ch. 21 - Prob. 52RECh. 21 - Prob. 53RECh. 21 - Prob. 54RECh. 21 - RECALL What is the connection between insulin and...
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