Biochemistry
9th Edition
ISBN: 9781305961135
Author: Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher: Cengage Learning
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Textbook Question
Chapter 2, Problem 56RE
RECALL What quality of zwitterions makes them desirable buffers?
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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism
of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help
To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?
To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!
Chapter 2 Solutions
Biochemistry
Ch. 2 - REFLECT AND APPLY Why is water necessary for life?Ch. 2 - REFLECT AND APPLY Contemplate biochemistry if...Ch. 2 - RECALL What is a van der Waals force?Ch. 2 - RECALL What is an induced dipole?Ch. 2 - RECALL What is a salt bridge?Ch. 2 - RECALL Under what circumstance is a molecule that...Ch. 2 - REFLECT AND APPLY Which would you think would be a...Ch. 2 - RECALL List the three types of van der Waals...Ch. 2 - RECALL A hydrogen bond is a special case of what...Ch. 2 - REFLECT AND APPLY Why do you think that most...
Ch. 2 - RECALL What are some macromolecules that have...Ch. 2 - BIOCHEMICAL CONNECTIONS How are hydrogen bonds...Ch. 2 - REFLECT AND APPLY Rationalize the fact that...Ch. 2 - REFLECT AND APPLY Draw three examples of types of...Ch. 2 - RECALL What are the requirements for molecules to...Ch. 2 - REFLECT AND APPLY Many properties of acetic acid...Ch. 2 - REFLECT AND APPLY How many water molecules could...Ch. 2 - REFLECT AND APPLY Both RNA and DNA have negatively...Ch. 2 - RECALL Identify the conjugate acids and bases in...Ch. 2 - RECALL Identify conjugate acids and bases in the...Ch. 2 - REFLECT AND APPLY Aspirin is an acid with a pKa of...Ch. 2 - RECALL Why does the pH change by one unit if the...Ch. 2 - MATHEMATICAL Calculate the hydrogen ion...Ch. 2 - MATHEMATICAL Calculate the hydrogen ion...Ch. 2 - MATHEMATICAL Calculate the hydroxide ion...Ch. 2 - RECALL Define the following: (a) Acid dissociation...Ch. 2 - REFLECT AND APPLY Look at Figure 2.17. If you did...Ch. 2 - BIOCHEMICAL CONNECTIONS List the criteria used to...Ch. 2 - BIOCHEMICAL CONNECTIONS What is the relationship...Ch. 2 - MATHEMATICAL What is the [CH3COO]/[CH3COOH] ratio...Ch. 2 - MATHEMATICAL What is the [CH3COO]/[CH3COOH] ratio...Ch. 2 - MATHEMATICAL What is the ratio of TRIS/TRIS-H+ in...Ch. 2 - MATHEMATICAL What is the ratio of HEPES/HEPES-H+...Ch. 2 - MATHEMATICAL How would you prepare 1 L of a 0.050...Ch. 2 - MATHEMATICAL The buffer needed for Question 35 can...Ch. 2 - MATHEMATICAL Calculate the pH of a buffer solution...Ch. 2 - MATHEMATICAL Calculate the pH of a buffer solution...Ch. 2 - MATHEMATICAL Calculate the pH of a buffer solution...Ch. 2 - MATHEMATICAL A catalog in the lab has a recipe for...Ch. 2 - MATHEMATICAL If you mix equal volumes of 0.1 M HCl...Ch. 2 - MATHEMATICAL What would be the pH of the solution...Ch. 2 - MATHEMATICAL If you have 100 mL of a 0.10 M TRIS...Ch. 2 - MATHEMATICAL What would be the pH of the solution...Ch. 2 - MATHEMATICAL Show that, for a pure weak acid in...Ch. 2 - MATHEMATICAL What is the ratio of concentrations...Ch. 2 - BIOCHEMICAL CONNECTIONS You need to carry out an...Ch. 2 - Prob. 48RECh. 2 - Prob. 49RECh. 2 - BIOCHEMICAL CONNECTIONS Which of the buffers shown...Ch. 2 - Prob. 51RECh. 2 - REFLECT AND APPLY In Section 2-4, we said that at...Ch. 2 - MATHEMATICAL Define buffering capacity. How do the...Ch. 2 - BIOCHEMICAL CONNECTIONS If you wanted to make a...Ch. 2 - BIOCHEMICAL CONNECTIONS We usually say that a...Ch. 2 - RECALL What quality of zwitterions makes them...Ch. 2 - Prob. 57RECh. 2 - Prob. 58RECh. 2 - Prob. 59RECh. 2 - BIOCHEMICAL CONNECTIONS A frequently recommended...
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