Biochemistry
9th Edition
ISBN: 9781305961135
Author: Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher: Cengage Learning
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Textbook Question
Chapter 2, Problem 37RE
MATHEMATICAL Calculate the pH of a buffer solution prepared by mixing 75 mL of 1.0 M lactic acid (see Table 2.6) and 25 mL of 1.0 M sodium lactate.
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Chapter 2 Solutions
Biochemistry
Ch. 2 - REFLECT AND APPLY Why is water necessary for life?Ch. 2 - REFLECT AND APPLY Contemplate biochemistry if...Ch. 2 - RECALL What is a van der Waals force?Ch. 2 - RECALL What is an induced dipole?Ch. 2 - RECALL What is a salt bridge?Ch. 2 - RECALL Under what circumstance is a molecule that...Ch. 2 - REFLECT AND APPLY Which would you think would be a...Ch. 2 - RECALL List the three types of van der Waals...Ch. 2 - RECALL A hydrogen bond is a special case of what...Ch. 2 - REFLECT AND APPLY Why do you think that most...
Ch. 2 - RECALL What are some macromolecules that have...Ch. 2 - BIOCHEMICAL CONNECTIONS How are hydrogen bonds...Ch. 2 - REFLECT AND APPLY Rationalize the fact that...Ch. 2 - REFLECT AND APPLY Draw three examples of types of...Ch. 2 - RECALL What are the requirements for molecules to...Ch. 2 - REFLECT AND APPLY Many properties of acetic acid...Ch. 2 - REFLECT AND APPLY How many water molecules could...Ch. 2 - REFLECT AND APPLY Both RNA and DNA have negatively...Ch. 2 - RECALL Identify the conjugate acids and bases in...Ch. 2 - RECALL Identify conjugate acids and bases in the...Ch. 2 - REFLECT AND APPLY Aspirin is an acid with a pKa of...Ch. 2 - RECALL Why does the pH change by one unit if the...Ch. 2 - MATHEMATICAL Calculate the hydrogen ion...Ch. 2 - MATHEMATICAL Calculate the hydrogen ion...Ch. 2 - MATHEMATICAL Calculate the hydroxide ion...Ch. 2 - RECALL Define the following: (a) Acid dissociation...Ch. 2 - REFLECT AND APPLY Look at Figure 2.17. If you did...Ch. 2 - BIOCHEMICAL CONNECTIONS List the criteria used to...Ch. 2 - BIOCHEMICAL CONNECTIONS What is the relationship...Ch. 2 - MATHEMATICAL What is the [CH3COO]/[CH3COOH] ratio...Ch. 2 - MATHEMATICAL What is the [CH3COO]/[CH3COOH] ratio...Ch. 2 - MATHEMATICAL What is the ratio of TRIS/TRIS-H+ in...Ch. 2 - MATHEMATICAL What is the ratio of HEPES/HEPES-H+...Ch. 2 - MATHEMATICAL How would you prepare 1 L of a 0.050...Ch. 2 - MATHEMATICAL The buffer needed for Question 35 can...Ch. 2 - MATHEMATICAL Calculate the pH of a buffer solution...Ch. 2 - MATHEMATICAL Calculate the pH of a buffer solution...Ch. 2 - MATHEMATICAL Calculate the pH of a buffer solution...Ch. 2 - MATHEMATICAL A catalog in the lab has a recipe for...Ch. 2 - MATHEMATICAL If you mix equal volumes of 0.1 M HCl...Ch. 2 - MATHEMATICAL What would be the pH of the solution...Ch. 2 - MATHEMATICAL If you have 100 mL of a 0.10 M TRIS...Ch. 2 - MATHEMATICAL What would be the pH of the solution...Ch. 2 - MATHEMATICAL Show that, for a pure weak acid in...Ch. 2 - MATHEMATICAL What is the ratio of concentrations...Ch. 2 - BIOCHEMICAL CONNECTIONS You need to carry out an...Ch. 2 - Prob. 48RECh. 2 - Prob. 49RECh. 2 - BIOCHEMICAL CONNECTIONS Which of the buffers shown...Ch. 2 - Prob. 51RECh. 2 - REFLECT AND APPLY In Section 2-4, we said that at...Ch. 2 - MATHEMATICAL Define buffering capacity. How do the...Ch. 2 - BIOCHEMICAL CONNECTIONS If you wanted to make a...Ch. 2 - BIOCHEMICAL CONNECTIONS We usually say that a...Ch. 2 - RECALL What quality of zwitterions makes them...Ch. 2 - Prob. 57RECh. 2 - Prob. 58RECh. 2 - Prob. 59RECh. 2 - BIOCHEMICAL CONNECTIONS A frequently recommended...
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- REFLECT AND APPLY You are purifying a protein for the first time. You have solubilized it with homogenization in a blender followed by differential centrifugation. You wish to try ammonium sulfate precipitation as the next step. Knowing nothing beforehand about the amount of ammonium sulfate to add, design an experiment to find the proper concentration (% saturation) of ammonium sulfate to use.arrow_forwardREFLECT AND APPLY Suggest a reason why the cell membranes of bacteria grown at 20C tend to have a higher proportion of unsaturated fatty acids than the membranes of bacteria of the same species grown at 37C. In other words, the bacteria grown at 37C have a higher proportion of saturated fatty acids in their cell membranes.arrow_forwardREFLECT AND APPLY Design an experiment to purify protein X on an anion-exchange column. Protein X has an isoelectric point of 7.0.arrow_forward
- REFLECT AND APPLY Referring to Question 23, how would you purify protein X using ion-exchange chromatography if it turns out the protein is only stable at a pH between 6 and 6.5?arrow_forwardREFLECT AND APPLY In reverse-phase HPLC, the stationary phase is nonpolar and the mobile phase is a polar solvent at neutral pH. Which of the three amino acids in Question 28 will move fastest on a reverse-phase HPLC column? Which one will move the slowest?arrow_forwardREFLECT AND APPLY Suggest a reason why the same protein system moves both sodium and potassium ions into and out of the cell.arrow_forward
- MATHEMATICAL Calculate the pH of a buffer solution that contains 0.10 M acetic acid (Table 2.6) and 0.25 M sodium acetate.arrow_forwardREFLECT AND APPLY Comment on the energetics of protein folding in light of the information in this chapter.arrow_forwardREFLECT AND APPLY The process of protein folding is spontaneous in the thermodynamic sense. It gives rise to a highly ordered conformation that has a lower entropy than the unfolded protein. How can this be?arrow_forward
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