ACHIEVE:INTRO TO GENETIC ANALYSIS 1TERM
12th Edition
ISBN: 9781319401399
Author: Griffiths
Publisher: MAC HIGHER
expand_more
expand_more
format_list_bulleted
Question
Chapter 2, Problem 27P
Summary Introduction
To determine: The number of distinct DNA bands that would be visible in each species if saparated by gel electrophoresis.
Introduction: Gel electrophoresis is a technique for detachment and study of macromolecules and fragments, based on the size and charge.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
Below are two pictures of perithecium on microscope slides that have been squished under cover slips so that the asci inside have spread
out. In the asci, we can clearly see the tan ascospores (light colored) and wildtype ascospores (dark colored). We will perform our tetrad
analysis by counting how many of the asci are non-recombinant and how many of the acsi are recombinant. We can tell which are
recombinant and non-recombinant by observing the patterns of the ascospores in the asci. See the patterns of ascospores for non-
recombinant and recombinant asci in the table below.
Ascospore pattern of
non-recombinant asci
OOOO
OOOOO
You will count the total number of non-recombinant and recombinant asci in the two pictures below. You will only count the asci that
have a star next to them because these asci can be clearly identified as non-recombinant or recombinant. Select the correct number
below after you are done.
Total number of non-recombinant asci [Select]
Ascospore pattern of…
A blood stain from a crime scene and blood samples from four suspects were analyzed by PCR using fluorescent primers associated with three STR loci: D3S1358, vWA, and FGA. The resulting electrophoretograms are shown below. The numbers beneath each peak identify the allele (upper box) and the height of the peak in relative fluorescence units (lower box). Solve, (a) Since everyone has two copies of each chromosome and therefore, two alleles of each gene, what accounts for the appearance ofonly one allele at some loci? (b) Which suspect is a possible source of the blood? (c) Could the suspect be identifi ed using just one of the three STR loci? (d) What can you conclude about the amount of DNA obtained from Suspect 1 compared to Suspect 4?
You cross two yeast strains one is an ade auxotroph the other is a pro auxotroph and allow
the diploid to sporulate. When you score each spore in the ascus you find the following
proportions: 518 PD, 8 NPD, and 225 T.
a.) What are the genotypes of each spore in all three types of the tetrads.
b) Are these genes linked why or why not?
c.) If these genes are unlinked what would you expect the progeny numbers and ratios to
be?
d.) What is the formula to determine the most accurate distance between these genes? If
linked what is the map distance?
Chapter 2 Solutions
ACHIEVE:INTRO TO GENETIC ANALYSIS 1TERM
Ch. 2 - Prob. 1PCh. 2 - Prob. 2PCh. 2 - Prob. 3PCh. 2 - Prob. 4PCh. 2 - Prob. 5PCh. 2 - Prob. 6PCh. 2 - Prob. 7PCh. 2 - Prob. 8PCh. 2 - Prob. 9PCh. 2 - Prob. 10P
Ch. 2 - Prob. 11PCh. 2 - Prob. 12PCh. 2 - Prob. 13PCh. 2 - Prob. 14PCh. 2 - Prob. 15PCh. 2 - Prob. 17PCh. 2 - Prob. 18PCh. 2 - Prob. 19PCh. 2 - Prob. 20PCh. 2 - Prob. 21PCh. 2 - Prob. 22PCh. 2 - Prob. 23PCh. 2 - Prob. 24PCh. 2 - Prob. 25PCh. 2 - Prob. 26PCh. 2 - Prob. 27PCh. 2 - Prob. 28PCh. 2 - Prob. 29PCh. 2 - Prob. 30PCh. 2 - Prob. 31PCh. 2 - Prob. 32PCh. 2 - Prob. 33PCh. 2 - Prob. 34PCh. 2 - Prob. 35PCh. 2 - Prob. 36PCh. 2 - Prob. 37PCh. 2 - Prob. 38PCh. 2 - Prob. 39PCh. 2 - Prob. 40PCh. 2 - Prob. 41PCh. 2 - Prob. 42PCh. 2 - Prob. 43PCh. 2 - Prob. 44PCh. 2 - Prob. 45PCh. 2 - Prob. 46PCh. 2 - Prob. 47PCh. 2 - Prob. 48PCh. 2 - Prob. 49PCh. 2 - Prob. 50PCh. 2 - Prob. 51PCh. 2 - Prob. 52PCh. 2 - Prob. 53PCh. 2 - Prob. 54PCh. 2 - Prob. 55PCh. 2 - Prob. 56PCh. 2 - Prob. 56.1PCh. 2 - Prob. 56.2PCh. 2 - Prob. 56.3PCh. 2 - Prob. 56.4PCh. 2 - Prob. 56.5PCh. 2 - Prob. 56.6PCh. 2 - Prob. 56.7PCh. 2 - Prob. 56.8PCh. 2 - Prob. 56.9PCh. 2 - Prob. 56.10PCh. 2 - Prob. 56.11PCh. 2 - Prob. 56.12PCh. 2 - Prob. 56.13PCh. 2 - Prob. 56.14PCh. 2 - Prob. 56.15PCh. 2 - Prob. 57PCh. 2 - Prob. 58PCh. 2 - Prob. 59PCh. 2 - Prob. 60PCh. 2 - Prob. 61PCh. 2 - Prob. 62PCh. 2 - Prob. 63PCh. 2 - Prob. 64PCh. 2 - Prob. 65PCh. 2 - Prob. 67PCh. 2 - Prob. 68PCh. 2 - Prob. 69PCh. 2 - Prob. 70PCh. 2 - Prob. 71PCh. 2 - Prob. 72PCh. 2 - Prob. 73PCh. 2 - Prob. 74PCh. 2 - Prob. 75PCh. 2 - Prob. 76PCh. 2 - Prob. 77PCh. 2 - Prob. 78PCh. 2 - Prob. 79PCh. 2 - Prob. 80PCh. 2 - Prob. 81PCh. 2 - Prob. 82PCh. 2 - Prob. 83PCh. 2 - Prob. 84PCh. 2 - Prob. 85PCh. 2 - Prob. 86PCh. 2 - Prob. 87PCh. 2 - Prob. 88PCh. 2 - Prob. 89PCh. 2 - Prob. 90PCh. 2 - Prob. 91PCh. 2 - Prob. 1GSCh. 2 - Prob. 2GSCh. 2 - Prob. 3GS
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Figure 1 is a photography obtained after spreading the replicating Escherichia coli chromosome and its observation by transmission electron microscopy. An interpretation scheme of the observed structure is shown in the upper right part of the photo. Figure 1: Photography of a replicating Escherichia coli chromosome observed DA by transmission electron microscopy. 2 to what do correspond respectively the grey, black and dotted lines? Indicate below the name of the chromosomal regions that are squared and named respectively A and B on Figure 1arrow_forwardIn the figure below, black and pink lines are used to represent nonhomologous chromosomes. Which of the figures matches the following descriptions? More than one diagram may correspond to any one statement, and a diagram may be a correct answer for more than one question. 1) Gametes produced by a reciprocal translocation heterozygote: 2) Genetically balanced gametes produced by a reciprocal translocation heterozygote:arrow_forwardNow that you have counted the non-recombinant and recombinant asci we want to determine the recombination frequency between the tan gene and the centromere. To calculate the recombination frequency we need an accurate count of the number of non-recombinant recombinant individuals. In the asci the individuals are the ascospores. and 1118 WWW 4 non- recombinant ascospores 4 non- recombinant ascospores 1838 W M M M 500 2 non- recombinant ascospores 2 recombinant ascospores 2 recombinant ascospores 2 non- recombinant ascospores Figure 1.6. The number of ascospores in non-recombinant and recombinant asci. In the non-recombinant asci all 8 ascospores are non-recombinant (see Figure 1.6 above). In the non-recombinant asci 4 of the ascospores are recombinant and 4 of the ascospores are non-recombinant (see Figure 1.6 above). To determine how many recombinant and non-recombinant ascospores where in the two pictures above we need to do the math below. You need to use the total number of…arrow_forward
- You and your lab partner performed a complementation test for five recessive histidine auxotrophs. Your plate looks like this: 1 2 3 4 5 1 2 3 4 5 growth no growth What do the gray patches represent ✓ [Select ] What do the tan patches represent? diploid histidine auxotrophs diploid prototrophs haploid histidine auxotrophs haploid prototrophs What is the composition of the medium for this test? [Select] The data contains a conflicting result due to a false positive (that is, it showed growth where it should not have grown) because your partner inoculated a patch too heavily. Which cross most likely led to this result? [Select] How many complementation groups are described by this data, taking into account that a cross yielded a false positive result? [Select]arrow_forwardImagine that you have done a cross between two strains of yeast, one of which has the genotype A B C and the other a b c, where the letters refer to three closely linked genes in the order given. You examine many tetrads resulting from this cross, and the two tetrads below are found that have atypical scoring patterns. In tetrad I, the spores are A B C, ABC, a B c, and a b c. In tetrad II, the spores are A B C, A b c, a b C, and a b c. Which tetrad or tetrads indicate(s) that gene conversion has occurred AND show(s) that recombination has occurred between genes A and C? O neither tetrads I and II O tetrad II O tetrad I O both tetrads I and IIarrow_forwardThe presence (+) or absence (−) of six sequences in each of five bacterial artificial chromosome (BAC) clones (A–E) is indicated in the following table. Using these markers, put the BAC clones in their correct order and indicate the locations of the numbered sequences within them. Sequences BAC clone 1 2 3 4 5 6 A + − − − + − B − − − + − + C − + + − − − D − − + − + − E + − − + − −arrow_forward
- Three haploid fungal mutants that require compound W for growth were isolated. Each mutant contains a recessive allele in a single gene. Three compounds (A, B and C) in the biosynthetic pathway to W are known, but their order in the pathway is unknown. Each compound is tested for its ability to support the growth of each of the three mutants. Phenotypes of all of the three mutants are shown in the following table (“+" indicates growth, "-" indicates no growth). A C W Mutant 1 Mutant 2 Mutant 3 What would be the phenotype of a haploid mutant that contains both mutant alleles in mutant 2 and 3? Phenotype refers to growth or absence of growth on compounds A, B, C and WN. O Like mutant 1 O Like mutant 2 Like mutant 3 O Like wild typearrow_forwardThe picture shows a gel of PCR products for the loci SRY, AMELX/Y and ZFX/Y, for three controls and five test human individuals (1-5). Below the gel is the phenotypic sex of each individual, their fertility status, and the number of Barr bodies seen in the nucleus of a somatic cell in each case. The diagram to the right shows the positions of the tested loci on the human sex chromosomes, and also the positions of the PRKX and PRKYgenes. Which sample carries a derived X chromosome with a translocation due to ectopic crossover between the loci PRKX and PRKY: 46,X,derX,t(X,Y); ? 1 2 3 4 5arrow_forwardThe diagram below represents results of agarose gel electrophoresis performed after PCR amplification of a molecular marker in diploid organisms. Answer the following questions: a) How many individuals are homozygous and how many are heterozygous? b) How many alleles are there in this population?arrow_forward
- In Figure 3-11, if the input genotypes were a • B and A • b,what would be the genotypes colored blue?arrow_forwardJA yeast proline, histidine auxotroph is mated with a prototroph and the diploid cell is sporulated. a.) Write out the cross that generated the diploid cell? b. ) List the types of tetrads and the genotypes of each spore in the tetrad that will occur if this diploid cell is sporulated.arrow_forwardAlleles of genes A and B were analyzed in Neurospora according to the cross shown below. Ordered tetrads are summarized in each horizontal row with the number of tetrads in each category listed alongside. A) Analyze the data to determine the recombination frequency (RF) between A and B, along with any additional information that is available from this data. B) Draw a map of the chromosome or chromosomes with appropriate map distances. C) Use the Perkins formula to reanalyze any relationship between A and B.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
DNA Use In Forensic Science; Author: DeBacco University;https://www.youtube.com/watch?v=2YIG3lUP-74;License: Standard YouTube License, CC-BY
Analysing forensic evidence | The Laboratory; Author: Wellcome Collection;https://www.youtube.com/watch?v=68Y-OamcTJ8;License: Standard YouTube License, CC-BY