Human Anatomy & Physiology Plus Mastering A&P with Pearson eText -- Access Card Package (2nd Edition) (What's New in Anatomy & Physiology)
2nd Edition
ISBN: 9780134702339
Author: Erin C. Amerman
Publisher: PEARSON
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Chapter 19.5, Problem 11QC
Summary Introduction
To review:
The role of prostacyclin in the regulation of hemostasis.
Introduction:
Hemostasis is a process in which bleeding is stopped from wounds. During the process, the wound and bleeding attracts platelets to aggregate and convert into large groups. Then the platelets move to the injured location and gather around the bleeding blood vessel. The platelets cause the blood to thicken and turn from a liquid, to a gel form, then the damaged blood vessel is repaired.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 19 Solutions
Human Anatomy & Physiology Plus Mastering A&P with Pearson eText -- Access Card Package (2nd Edition) (What's New in Anatomy & Physiology)
Ch. 19.1 - Prob. 1QCCh. 19.1 - Prob. 2QCCh. 19.1 - 3. How does blood regulate temperature and...Ch. 19.1 - 4. What are the four main categories of plasma...Ch. 19.1 - Prob. 5QCCh. 19.2 - Describe the structure of a typical erythrocyte.Ch. 19.2 - Prob. 2QCCh. 19.2 - 3. Walk through the basic steps of...Ch. 19.2 - 4. What is the lifespan of an erythrocyte?
Ch. 19.2 - Prob. 5QC
Ch. 19.2 - Prob. 6QCCh. 19.2 - Prob. 7QCCh. 19.3 - How do granulocytes and agranulocytes differ?Ch. 19.3 - Prob. 2QCCh. 19.3 - Compare the two types of agranulocytes.Ch. 19.3 - 4. How do B and T lymphocytes differ?
Ch. 19.3 - 5. What does a monocyte become in the tissues?...Ch. 19.3 - Prob. 6QCCh. 19.3 - 7. How do the lymphoid and myeloid cell lines...Ch. 19.3 - 8. Where do T and B lymphocytes mature?
Ch. 19.4 - Prob. 1QCCh. 19.4 - Prob. 2QCCh. 19.5 - Prob. 1QCCh. 19.5 - Prob. 2QCCh. 19.5 - 3. How are platelets triggered to aggregate?
Ch. 19.5 - Prob. 4QCCh. 19.5 - How do the intrinsic/contact activation and...Ch. 19.5 - Prob. 6QCCh. 19.5 - Prob. 7QCCh. 19.5 - What is thrombolysis?Ch. 19.5 - Prob. 9QCCh. 19.5 - Prob. 10QCCh. 19.5 - Prob. 11QCCh. 19.5 - Prob. 12QCCh. 19.5 - Prob. 13QCCh. 19.5 - Prob. 14QCCh. 19.6 - Prob. 1QCCh. 19.6 - Prob. 2QCCh. 19.6 - Prob. 3QCCh. 19.6 - Prob. 4QCCh. 19.6 - Prob. 5QCCh. 19 - Prob. 1CYRCh. 19 - Prob. 2CYRCh. 19 - List the seven major functions of blood.Ch. 19 - Prob. 4CYRCh. 19 - Prob. 5CYRCh. 19 - Prob. 6CYRCh. 19 - Prob. 7CYRCh. 19 - Leukocytes are: a. nucleated cells that function...Ch. 19 - Prob. 9CYRCh. 19 - Fill in the blanks: Lymphocytes are derived from...Ch. 19 - Prob. 11CYRCh. 19 - Number the steps of hemostasis in order, putting 1...Ch. 19 - Prob. 13CYRCh. 19 - Prob. 14CYRCh. 19 - Prob. 15CYRCh. 19 - Prob. 16CYRCh. 19 - Prob. 17CYRCh. 19 - 18. Which antigens does a person with blood type...Ch. 19 - Prob. 19CYRCh. 19 - Prob. 20CYRCh. 19 - 1. Explain how blood, being a liquid, enables all...Ch. 19 - Prob. 2CYUCh. 19 - 3. The anticoagulant drug warfarin primarily...Ch. 19 - 4. Cirrhosis of the liver often reduces production...Ch. 19 - 1. A blood sample from your patient shows that she...Ch. 19 - Prob. 2AYKACh. 19 - Prob. 3AYKACh. 19 - Prob. 4AYKB
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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