You have a bacterial strain with the CMU operon: a) As shown in the image below, the cmu operon encodes a peptide (Pep1), as well as a kinase and regulator corresponding to a two-component system. The cmu operon is activated when Pep 1 is added to the growth media. Pep1 is a peptide that when added extracellularly leads to activation of the Cmu operon. Pep1 cmu-kinase cmu-regulator You also have these genetic components in other strains: b) An alternative sigma factor, with a promoter activated by the cmu-regulator, that control a series of multiple operons that together encode a transformasome (cellular machinery for transformation). c) the gene cl (a repressor). d) the promoter X, which includes a cl binding site (and in the absence of cl is active). e) the gene gp (encoding a green fluorescence protein). Using the cmu operon as a starting point, and assuming you can perform cloning to rearrange any of these genomic features, how would you use one or more of these to modify the bacteria for the following goals: Note: Your answers need to include the genes, promoters, transcriptional terminators and their order. Don't worry about components required for translation. 1. Draw the operon(s) required for a bacterial cell that produces GFP when Pep1 is added to the media 2. Draw the operon(s) required for a bacterial cell that takes up DNA when the environment when Pep1 is added to the media 3. Draw the operon(s) required for a bacterial cell that produces GFP in cells that are not exposed to Pep1 but inhibits GFP synthesis when Pep1 is added to the media.
You have a bacterial strain with the CMU operon: a) As shown in the image below, the cmu operon encodes a peptide (Pep1), as well as a kinase and regulator corresponding to a two-component system. The cmu operon is activated when Pep 1 is added to the growth media. Pep1 is a peptide that when added extracellularly leads to activation of the Cmu operon. Pep1 cmu-kinase cmu-regulator You also have these genetic components in other strains: b) An alternative sigma factor, with a promoter activated by the cmu-regulator, that control a series of multiple operons that together encode a transformasome (cellular machinery for transformation). c) the gene cl (a repressor). d) the promoter X, which includes a cl binding site (and in the absence of cl is active). e) the gene gp (encoding a green fluorescence protein). Using the cmu operon as a starting point, and assuming you can perform cloning to rearrange any of these genomic features, how would you use one or more of these to modify the bacteria for the following goals: Note: Your answers need to include the genes, promoters, transcriptional terminators and their order. Don't worry about components required for translation. 1. Draw the operon(s) required for a bacterial cell that produces GFP when Pep1 is added to the media 2. Draw the operon(s) required for a bacterial cell that takes up DNA when the environment when Pep1 is added to the media 3. Draw the operon(s) required for a bacterial cell that produces GFP in cells that are not exposed to Pep1 but inhibits GFP synthesis when Pep1 is added to the media.
Biology (MindTap Course List)
11th Edition
ISBN:9781337392938
Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Chapter14: Gene Regulation
Section: Chapter Questions
Problem 13TYU: INTERPRET DATA Develop a simple hypothesis that would explain the behavior of each of the following...
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Transcribed Image Text:You have a bacterial strain with the CMU operon:
a) As shown in the image below, the cmu operon encodes a peptide (Pep1), as well as a
kinase and regulator corresponding to a two-component system. The cmu operon is
activated when Pep 1 is added to the growth media.
Pep1 is a peptide that when added extracellularly leads to activation of the Cmu operon.
Pep1 cmu-kinase cmu-regulator
You also have these genetic components in other strains:
b) An alternative sigma factor, with a promoter activated by the cmu-regulator, that control a
series of multiple operons that together encode a transformasome (cellular machinery
for transformation).
c) the gene cl (a repressor).
d) the promoter X, which includes a cl binding site (and in the absence of cl is active).
e) the gene gp (encoding a green fluorescence protein).
Using the cmu operon as a starting point, and assuming you can perform cloning to rearrange
any of these genomic features, how would you use one or more of these to modify the bacteria
for the following goals:
Note: Your answers need to include the genes, promoters, transcriptional terminators and their
order. Don't worry about components required for translation.
1. Draw the operon(s) required for a bacterial cell that produces GFP when Pep1 is
added to the media
2. Draw the operon(s) required for a bacterial cell that takes up DNA when the
environment when Pep1 is added to the media
3. Draw the operon(s) required for a bacterial cell that produces GFP in cells that are
not exposed to Pep1 but inhibits GFP synthesis when Pep1 is added to the media.
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