Human Anatomy & Physiology Plus Mastering A&P with Pearson eText -- Access Card Package (2nd Edition) (What's New in Anatomy & Physiology)
2nd Edition
ISBN: 9780134702339
Author: Erin C. Amerman
Publisher: PEARSON
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Textbook Question
Chapter 19.3, Problem 5QC
What does a monocyte become in the tissues? What is its primary function?
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 19 Solutions
Human Anatomy & Physiology Plus Mastering A&P with Pearson eText -- Access Card Package (2nd Edition) (What's New in Anatomy & Physiology)
Ch. 19.1 - Prob. 1QCCh. 19.1 - Prob. 2QCCh. 19.1 - 3. How does blood regulate temperature and...Ch. 19.1 - 4. What are the four main categories of plasma...Ch. 19.1 - Prob. 5QCCh. 19.2 - Describe the structure of a typical erythrocyte.Ch. 19.2 - Prob. 2QCCh. 19.2 - 3. Walk through the basic steps of...Ch. 19.2 - 4. What is the lifespan of an erythrocyte?
Ch. 19.2 - Prob. 5QC
Ch. 19.2 - Prob. 6QCCh. 19.2 - Prob. 7QCCh. 19.3 - How do granulocytes and agranulocytes differ?Ch. 19.3 - Prob. 2QCCh. 19.3 - Compare the two types of agranulocytes.Ch. 19.3 - 4. How do B and T lymphocytes differ?
Ch. 19.3 - 5. What does a monocyte become in the tissues?...Ch. 19.3 - Prob. 6QCCh. 19.3 - 7. How do the lymphoid and myeloid cell lines...Ch. 19.3 - 8. Where do T and B lymphocytes mature?
Ch. 19.4 - Prob. 1QCCh. 19.4 - Prob. 2QCCh. 19.5 - Prob. 1QCCh. 19.5 - Prob. 2QCCh. 19.5 - 3. How are platelets triggered to aggregate?
Ch. 19.5 - Prob. 4QCCh. 19.5 - How do the intrinsic/contact activation and...Ch. 19.5 - Prob. 6QCCh. 19.5 - Prob. 7QCCh. 19.5 - What is thrombolysis?Ch. 19.5 - Prob. 9QCCh. 19.5 - Prob. 10QCCh. 19.5 - Prob. 11QCCh. 19.5 - Prob. 12QCCh. 19.5 - Prob. 13QCCh. 19.5 - Prob. 14QCCh. 19.6 - Prob. 1QCCh. 19.6 - Prob. 2QCCh. 19.6 - Prob. 3QCCh. 19.6 - Prob. 4QCCh. 19.6 - Prob. 5QCCh. 19 - Prob. 1CYRCh. 19 - Prob. 2CYRCh. 19 - List the seven major functions of blood.Ch. 19 - Prob. 4CYRCh. 19 - Prob. 5CYRCh. 19 - Prob. 6CYRCh. 19 - Prob. 7CYRCh. 19 - Leukocytes are: a. nucleated cells that function...Ch. 19 - Prob. 9CYRCh. 19 - Fill in the blanks: Lymphocytes are derived from...Ch. 19 - Prob. 11CYRCh. 19 - Number the steps of hemostasis in order, putting 1...Ch. 19 - Prob. 13CYRCh. 19 - Prob. 14CYRCh. 19 - Prob. 15CYRCh. 19 - Prob. 16CYRCh. 19 - Prob. 17CYRCh. 19 - 18. Which antigens does a person with blood type...Ch. 19 - Prob. 19CYRCh. 19 - Prob. 20CYRCh. 19 - 1. Explain how blood, being a liquid, enables all...Ch. 19 - Prob. 2CYUCh. 19 - 3. The anticoagulant drug warfarin primarily...Ch. 19 - 4. Cirrhosis of the liver often reduces production...Ch. 19 - 1. A blood sample from your patient shows that she...Ch. 19 - Prob. 2AYKACh. 19 - Prob. 3AYKACh. 19 - Prob. 4AYKB
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