Campbell Biology Custom Stony Brook 10 Th Edition
Campbell Biology Custom Stony Brook 10 Th Edition
10th Edition
ISBN: 9781269870818
Author: Reece Urry Cain Wasserman Minorsky Jackson
Publisher: PEARSON
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Chapter 19, Problem 4TYU
Summary Introduction

Introduction: Bacteria are single-celled prokaryotic microorganisms. A bacteriophage is also called the bacterial virus that is formed when a virus infects a bacterium. T2 and T4 are enterobacteria phages that infect and kill E. coli. They are also known as T-even phages because they are double stranded. T-even phages are commonly used as model organisms for various genetic studies. The experiments performed by Hershey and Chase using bacteriophages already proved that DNA is the genetic material of inheritance.

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Overview of Transformation Protocol   -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes.   Questions: 1) What differences would you expect to see between the…
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