Campbell Biology Custom Stony Brook 10 Th Edition
10th Edition
ISBN: 9781269870818
Author: Reece Urry Cain Wasserman Minorsky Jackson
Publisher: PEARSON
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Chapter 19.2, Problem 2CC
Summary Introduction
To compare: Viral and cellular RNA polymerase in terms of template and overall functions.
Concept introduction: RNA polymerase is an enzyme found in all organisms. Viral RNA polymerases play an important role in replication and transcription of virus. Replication is a process of synthesizing an exact copy of genetic material (DNA or RNA) from the parent or template strand. Transcription is a process where RNA is formed using DNA or RNA as a template.
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Overview of Transformation Protocol
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Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
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Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 19 Solutions
Campbell Biology Custom Stony Brook 10 Th Edition
Ch. 19.1 - Prob. 1CCCh. 19.1 - MAKE CONNECTIONS Bacteriophages were used to...Ch. 19.2 - Compare the effect on the host cell of a lytic...Ch. 19.2 - Prob. 2CCCh. 19.2 - Why is HIV called a retrovirus?Ch. 19.2 - Prob. 4CCCh. 19.3 - Describe two ways in which a preexisting virus can...Ch. 19.3 - Contrast horizontal and vertical transmission of...Ch. 19.3 - WHAT IF? TMV has been isolated from virtually all...Ch. 19 - Are viruses generally considered living or...
Ch. 19 - Prob. 19.2CRCh. 19 - Prob. 19.3CRCh. 19 - Which of the following characteristics,...Ch. 19 - Emerging viruses arise by (A) mutation of existing...Ch. 19 - Prob. 3TYUCh. 19 - Prob. 4TYUCh. 19 - Prob. 5TYUCh. 19 - Prob. 6TYUCh. 19 - EVOLUTION CONNECTION The successof some viruses...Ch. 19 - SCIENTIFIC INQUIRY When bacteria infect an animal,...Ch. 19 - WRITE ABOUT A THEME: ORGANIZATION While viruses...Ch. 19 - Prob. 10TYU
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