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Biochemistry, The Molecular Basis of Life, 6th Edition
6th Edition
ISBN: 9780190259204
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 18, Problem 46RQ
Summary Introduction
To review:
The role of stalled RNA (ribonucleic acid) polymerase intranscription-couplednucleotide excision repair.
Introduction:
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Students have asked these similar questions
A protein biochemist attempted to determine the amino acid sequence of a decapeptide. The results from the trypsin,
chymotrypsin, and cyanogen bromide treatments are shown:
⚫ Trypsin digestion gave two fragments with multiple residues (not in order):
• T1: Ala, Arg, Phe, Gly, Thr, Trp, Tyr
o T2: Lys, Met, Val
Chymotrypsin digestion gave four fragments with multiple residues (not in order):
• CT1: Ala, Phe
• CT2: Thr, Trp
• CT3: Lys, Met, Tyr, Val
。 CT4: Arg, Gly
⚫ Treatment with cyanogen bromide yielded a single amino acid, methionine, and a nonapeptide.
What is a possible sequence of the decapeptide? Use three-letter abbreviations in your answer, and add a dash between
each residue.
A protein biochemist attempted to determine the amino acid sequence of a decapeptide. The results from the trypsin,
chymotrypsin, and cyanogen bromide treatments are shown:
• Trypsin digestion gave two fragments with multiple residues (not in order):
T1: Ala, Arg, Phe, Gly, Thr, Trp, Tyr
• T2: Lys, Met, Val
Chymotrypsin digestion gave four fragments with multiple residues (not in order):
• CTI: Ala, Phe
。 CT2: Thr, Trp
• CT3: Lys, Met, Tyr, Val
。 CT4: Arg, Gly
⚫ Treatment with cyanogen bromide yielded a single amino acid, methionine, and a nonapeptide.
What is a possible sequence of the decapeptide? Use three-letter abbreviations in your answer, and add a dash between
each residue.
Poly-L-leucine in an organic solvent such as dioxane is a helical, whereas poly-L-isoleucine is not.
Why do these amino acids with the same number and kinds of atoms have different helix-forming tendencies?
L-isoleucine's methyl group sterically interferes with α-helix formation, whereas L-leucine's methyl group does
not interfere.
L-isoleucine lacks an NH group that can donate a hydrogen bond to stabilize an a helix, whereas L-leucine has an
NH group.
L-isoleucine contains a hydrogen-bond acceptor in close proximity to the main chain, whereas L-leucine lacks
this group.
L-isoleucine's ring structure prevents it from assuming the op value to fit into an a helix, whereas L-isoleucine does
not have a ring structure.
Chapter 18 Solutions
Biochemistry, The Molecular Basis of Life, 6th Edition
Ch. 18 - Prob. 1QCh. 18 - Prob. 2QCh. 18 - Prob. 3QCh. 18 - Prob. 4QCh. 18 - Prob. 5QCh. 18 - Prob. 1RQCh. 18 - Prob. 2RQCh. 18 - Prob. 3RQCh. 18 - Prob. 4RQCh. 18 - Prob. 5RQ
Ch. 18 - Prob. 6RQCh. 18 - Prob. 7RQCh. 18 - Prob. 8RQCh. 18 - Prob. 9RQCh. 18 - Prob. 10RQCh. 18 - Prob. 11RQCh. 18 - Prob. 12RQCh. 18 - Prob. 13RQCh. 18 - Prob. 14RQCh. 18 - Prob. 15RQCh. 18 - Prob. 16RQCh. 18 - Prob. 17RQCh. 18 - Prob. 18RQCh. 18 - Prob. 19RQCh. 18 - Prob. 20RQCh. 18 - Prob. 21RQCh. 18 - Prob. 22RQCh. 18 - Prob. 23RQCh. 18 - Prob. 24RQCh. 18 - Prob. 25RQCh. 18 - Prob. 26RQCh. 18 - Prob. 27RQCh. 18 - Prob. 28RQCh. 18 - Prob. 29RQCh. 18 - Prob. 30RQCh. 18 - Prob. 31RQCh. 18 - Prob. 32RQCh. 18 - Prob. 33RQCh. 18 - Prob. 34RQCh. 18 - Prob. 35RQCh. 18 - Prob. 36RQCh. 18 - Prob. 37RQCh. 18 - Prob. 38RQCh. 18 - Prob. 39RQCh. 18 - Prob. 40RQCh. 18 - Prob. 41RQCh. 18 - Prob. 42RQCh. 18 - Prob. 43RQCh. 18 - Prob. 44RQCh. 18 - Prob. 45RQCh. 18 - Prob. 46RQCh. 18 - Prob. 47FBCh. 18 - Prob. 48FBCh. 18 - Prob. 49FBCh. 18 - Prob. 50FBCh. 18 - Prob. 51FBCh. 18 - Prob. 52FBCh. 18 - Prob. 53FBCh. 18 - Prob. 54FBCh. 18 - Prob. 55FBCh. 18 - Prob. 56FBCh. 18 - Prob. 57SACh. 18 - Prob. 58SACh. 18 - Prob. 59SACh. 18 - Prob. 60SACh. 18 - Prob. 61SACh. 18 - Prob. 62TQCh. 18 - Prob. 63TQCh. 18 - Prob. 64TQCh. 18 - Prob. 65TQCh. 18 - Prob. 66TQCh. 18 - Prob. 67TQCh. 18 - Prob. 68TQCh. 18 - Prob. 69TQCh. 18 - Prob. 70TQCh. 18 - Prob. 71TQCh. 18 - Prob. 72TQCh. 18 - Prob. 73TQCh. 18 - Prob. 74TQCh. 18 - Prob. 75TQ
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.Similar questions
- Samples of the octapeptide AVGWRVKS are subjected to proteolytic cleavage by either trypsin or chymotrypsin. Select the most appropriate technique for separating the digestion products of AVGWRVKS if cleaved by trypsin. salting out ion-exchange chromatography gel-filtration chromatography dialysis Select the most appropriate technique for separating the digestion products of AVGWRVKS if cleaved by chymotrypsin. ion-exchange chromatography gel-filtration chromatography dialysis salting outarrow_forwardClassify each description as corresponding to DNA or RNA. DNA a single molecule that can be over 10,000,000 nucleotides long Answer Bank usually single stranded RNA makes up the genome for eukaryotic organisms a single molecule that can form a complex secondary structure usually double stranded can be translated into a protein includes deoxyribose sugar includes the base uracilarrow_forwardA protein biochemist attempted to determine the amino acid sequence of a decapeptide. The results from the trypsin, chymotrypsin, and cyanogen bromide treatments are shown: • Trypsin digestion gave two fragments with multiple residues (not in order): T1: Ala, Arg, Phe, Gly, Thr, Trp, Tyr • T2: Lys, Met, Val ⚫ Chymotrypsin digestion gave four fragments with multiple residues (not in order): • CT1: Ala, Phe ° • CT2: Thr, Trp 。 CT3: Lys, Met, Tyr, Val • CT4: Arg, Gly ⚫ Treatment with cyanogen bromide yielded a single amino acid, methionine, and a nonapeptide. What is a possible sequence of the decapeptide? Use three-letter abbreviations in your answer, and add a dash between each residue. sequence:arrow_forward
- Classify each nucleotide or nucleoside by the type of base and sugar present in each image. HO. -CH₂ 0 NH₂ OH OH OH он NH Classify the molecule. Opurine, deoxyribose purine, ribose Opyrimidine, deoxyribose pyrimidine, ribose NH₂ Classify the molecule. purine, deoxyribose Opyrimidine, ribose Opurine, ribose pyrimidine, deoxyribose H₂C. ·CH₂ NH Classify the molecule. pyrimidine, ribose Opurine, deoxyribose O purine, ribose Opyrimidine, deoxyribose OHarrow_forwardWrite the complementary sequence for GACATC. Write the new sequence in the standard 5'-to-3' notation. 5'- -3'arrow_forwardSuppose that you are given a mixture of proteins, whose properties are provided in the table. Isoelectric point (pl) Molecular weight (in kDa) Protein A 4.1 80 Protein B 9.0 81 Protein C 8.8 37 Protein D 3.9 172 Select one combination of techniques that can be used to isolate Protein B from Proteins A, C, and D. gel filtration chromatography and ultracentrifugation dialysis and ultracentrifugation dialysis and ion exchange chromatography ion exchange chromatography and gel filtration chromatographyarrow_forward
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