Study Guide for Campbell Biology
Study Guide for Campbell Biology
11th Edition
ISBN: 9780134443775
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece, Martha R. Taylor, Michael A. Pollock
Publisher: PEARSON
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Chapter 18, Problem 2TYK
Summary Introduction

Introduction: In genetics, an operon is the functional unit of a gene. Operon has a cluster of genes controlled by single promoter. Trp operon is a characteristic feature of many bacteria including Escherichia coli (E. coli). It is a gram-negative, rod-shaped bacteria living in the large intestine of higher vertebrates. In E. coli, tryp operon is used for the transcription of genes responsible for the production of an amino acid tryptophan. Trp operon is an example of the negative feedback mechanism, where the presence of increased levels of tryptophan switches off the trp operon.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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QCE Biology: Introduction to Gene Expression; Author: Atomi;https://www.youtube.com/watch?v=a7hydUtCIJk;License: Standard YouTube License, CC-BY