Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 17, Problem 53SA
Summary Introduction
To review:
The elimination of the effects of BPA in Avy offspring when their parents were fed with methyl donor-richdiet.
Introduction:
It was observed that when mice with agouti coatcolorweresupplemented with bisphenolA (BPA), the coatcolor of the offspring shifted from brown to yellow. BPA is responsible for epigenetic changes that do not alter gene sequence but alters the gene expression rate. When BPAsupplemented mice were provided with methyl donors (foliate, choline and vitamin B12) in their lab chow, the effects of BPA (bisphenol A) were eliminated. The offspring produced from such mice tended to havebrown-colored coats again.
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A classic way to isolate thymidylate synthase-negative mutants of bacteria
is to treat a growing culture with thymidine and trimethoprim. Most of
the cells are killed, and the survivors are greatly enriched in thymidylate
synthase-negative mutants.
(a) What phenotype would allow you to identify these mutants?
(b) What is the biochemical rationale for the selection? (That is, why are the
mutants not killed under these conditions?)
(c) How would the procedure need to be modified to select mammalian cell
mutants defective in thymidylate synthase?
A classic way to isolate thymidylate synthase-negative mutants of
bacteria is to treat a growing culture with thymidine and trimetho-
prim. Most of the cells are killed, and the survivors are greatly
enriched in thymidylate synthase-negative mutants.
(a) What phenotype would allow you to identify these mutants?
(b) What is the biochemical rationale for the selection? (That is, why
are the mutants not killed under these conditions?)
(c) How would the procedure need to be modified to select mam-
malian cell mutants defective in thymidylate synthase?
Could you please help answer this question?
Chapter 17 Solutions
Biochemistry: The Molecular Basis of Life
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