Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 17, Problem 14RQ
Summary Introduction
To review:
Definition of the following terminologies:
1. Cistron
2. Operon
3.miRNA (micro ribonucleic Acid)
4.siRNA (small interfering RNA)
5.snoRNA (small nucleolar RNA)
Introduction:
The DNA (deoxyribonucleic acid) serves as the primary genetic constituent in most of the organisms. Certain genetic elements such as cistron and operons may be present in the DNA. Moreover, the DNA may code for noncoding RNA molecules such as miRNA, siRNA, and snoRNA.
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Question 2:
Part a: Complete the table describing different components of intron removal from mRNA. Nu:, X and Y refer to B-type chemistry shown on the previous page. (YELLOW table shown)
Part b: Complete the table describing different components of group I self-splicing intron removal from 26S rRNA in Tetrahymena. (BLUE table shown)
Part c: Draw the intron with an all atom structure for Branchpoint A after intron removal from mRNA
Part d: Draw the Group I self-splicing intron with an all atom structure for the Guanosine cofactor after intron removal from 26S rRNA in Tetrahymena.
Gene expression is initiated largely in response to changes in the ____________________________ in prokaryotes as opposed to changes affecting the homeostasis of the whole organism in eukaryotes.
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You are studying a biosynthetic pathway in E. coli. You isolate mutants
that can be grouped into 1 of 3 groups (mut1-3), with mut1 having a
mutation in gene 1, mut2 in gene 2.... Based on the results of the feeding
table, where "+" indicates growth and "-" indicates lack of growth, draw
the biochemical pathway indicating the correct sequence of the different
chemicals and the enzymes that catalyze the different steps.
supplements
no
Strain
Wild-type+
mut1
mut2
mut3
P
+
S
+
+
σ
+
+
+
R
+
+
+
+
Chapter 17 Solutions
Biochemistry: The Molecular Basis of Life
Ch. 17 - Prob. 1QCh. 17 - Prob. 2QCh. 17 - Prob. 3QCh. 17 - Prob. 4QCh. 17 - Prob. 5QCh. 17 - Prob. 6QCh. 17 - Prob. 7QCh. 17 - Prob. 8QCh. 17 - Prob. 1RQCh. 17 - Prob. 2RQ
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