Biochemistry: The Molecular Basis of Life
Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 17, Problem 68TQ
Summary Introduction

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The phenomenon of new diseases, formed or exacerbating of existing pathologies, as a result of the incorporation of the host genome into the viral structure during the infective process.

Introduction:

A virus is an obligate intracellular parasite. It is known to be a nonliving entity, when present in the environment outside its host. Once it infects the host, it becomes a living organism. There is a specific type of host for a particular virus. The virus uses the metabolic machinery of the host to produce multiple copies of itself, or virions.

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Draw a diagram to demonstrate 3' to 5' exonuclease activity?
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You were tasked with purifying an untagged transcription factor (molecular weight 65,000Da, isoelectric point unknown) from a contaminant Protein A (molecular weight 50,000 Da, isoelectric point 5.0). You were also instructed to use the protein for crystallization studies after purification.i) In the initial purification step, which type of chromatography should you attempt? Explain your choice and specify the requirements of the buffer solution you should use. ii) Analyzing the proteins recovered from step i) using SDS-PAGE, you still observed a faint band of Protein A in addition to the transcription factor band. Given the limited time available for further purification, you must choose ONE appropriate chromatography method to maximize the chances of separating the two proteins as well as your crystallization studies. Provide a detailed explanation of your selection and the techniques/strategies you would employ to achieve this.

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Biochemistry: The Molecular Basis of Life

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