Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN: 9781305389892
Author: Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher: Cengage Learning
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Chapter 15, Problem 13TYK
Summary Introduction
To review:
The importance of
Introduction:
Nucleic acids are defined as
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When answering the questions below, please use the ONE-LETTER CODE for the amino acid, with NO spaces and symbols between each letter.a. What is the C-terminal residue? b. What is the N-terminal residue? c. What is the sequence of Peptide 2? d. What is the sequence of Peptide 4? e. What is the overall amino acid sequence?
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Chapter 15 Solutions
Biology: The Dynamic Science (MindTap Course List)
Ch. 15.1 - Prob. 1SBCh. 15.1 - If the codon were five bases long, how many...Ch. 15.2 - For the DNA template below, what would be the...Ch. 15.2 - Prob. 2SBCh. 15.3 - Prob. 1SBCh. 15.3 - Prob. 2SBCh. 15.4 - Prob. 1SBCh. 15.4 - Distinguish between the P, A, and E sites of the...Ch. 15.4 - Prob. 3SBCh. 15.5 - How does a missense mutation differ from a silent...
Ch. 15.5 - Prob. 2SBCh. 15.5 - Prob. 3SBCh. 15.5 - Prob. 4SBCh. 15 - Eukaryotic mRNA: usessnRNPs to cut out introns and...Ch. 15 - A segment of a strand of DNA has a base sequence...Ch. 15 - Prob. 3TYKCh. 15 - Prob. 4TYKCh. 15 - Which of the following statements is false? a. GTP...Ch. 15 - Prob. 6TYKCh. 15 - Prob. 7TYKCh. 15 - Prob. 8TYKCh. 15 - Prob. 9TYKCh. 15 - A part of an mRNA molecule with the sequence 5-UGC...Ch. 15 - Discuss Concepts A mutation occurs that alters an...Ch. 15 - Discuss Concepts The normal form of a gene...Ch. 15 - Prob. 13TYKCh. 15 - Prob. 14TYKCh. 15 - Prob. 15TYKCh. 15 - Prob. 16TYKCh. 15 - Prob. 17TYKCh. 15 - Prob. 1ITD
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- The link between gene and protein was first articulated by Beadle & Tatum, who proposed the one-gene, one-enzyme hypothesis - which of the following statements contradicts this hypothesis? Sickle-cell anemia results in defective hemoglobin. Two enzymes are able to metabolize the same reaction. Alkaptonuria results when individuals lack a single enzyme involved in the catalysis of homogentisic acid. A mutation in a single gene can result in a defective protein. A single antibody gene can code for different related proteins, depending on the splicing that takes place post-transcriptionally.arrow_forwardWhether the statement "Intrinsically disordered regions of proteins can be identified using bioinformatics methods to search genes for encoded amino acid sequences that possess high hydrophobicity and low net charge" is true or false.arrow_forwardBiochemistry: Site-directed mutagenesis, in which individual amino acid residues are replaced with others, is a powerful method to study enzyme mechanisms. In experiments with particular enzyme, various lysine residues were replaced with aspartate, yielding the results summarized in the table below: Enzyme Form: Enzyme Activity (U/mg) Native enzyme: 1,000 U/mg Recombinant Lys 21 to Asp 21: 970 U/mg Recombinant Lys 86 to Asp 86: 100 U/mg Recombinant Lys 101 to Asp 101: 970 U/mg a. What might be inferred about the role of Lys 21, 86, and 101 in the catalytic mechanism of this enzyme? b. Discuss where within the enzyme one might find Lys 21 and 101. Are these residues likely to be evolutionary conserved in this enzyme? Explain c. Is Lys position 86 likely to be evolutionary conserved? Explainarrow_forward
- How does Foldit Protein Folding Game use puzzles like Design, De Novo, Revisiting, help in bioinformatics and visualizing protein structure?arrow_forwardSuppose that there is an unknown protein that underwent Edman sequencing method. From N- terminal determination, a biochemist found out that there are two N-terminal amino acid residues, V and G. What is the original sequence of the protein given the following peptide fragments: after digestion with Chymotrypsin: G-L-S-R-G-M-w V-A-L-F Q-L-Y L-R-V-W G-M-V-E-A-D-I-P K-S-P-E-M-T-W R-M-A-S-E-K-P-G-H after digestion with Trypsin: P-G-H V-W-G-M-V-E-A-D-I-P M-A-S-E-K G-M-W-Q-L-Y-L-R S-P-E-M-T-W-R G-L-S-R V-A-L-F-K after digestion with Cyanogen Bromide: T-W-R-M W-Q-L-Y-L-R-V-W-G-M V-E-A-D-I-P A-S-E-K-P-G-H V-A-L-F-K-S-P-E-M G-L-S-R-G-Marrow_forwardWe need a string-matching-based approach to identifying protein homologies between several proteins. In what way does it function? With what evidence do you back up your assertion?arrow_forward
- As described in the figure below, the technique of ultracentrifugation can be used to demonstrate whether a substance is composed of large covalently linked molecules, or smaller molecules held together in a complex by noncovalent bonds. You are studying two substances that both have very high molecular weights: hyaluronan, a carbohydrate found in the extracellular matrix with a MW of ~8 x 106 daltons, and the bacterial ribosome, with a MW of -2.5 x 106. Hyaluronan is a single, long-chain polymer, while the ribosome subunit is a complex of 55 different proteins plus three long RNA molecules. Which of the following describes the pattern you would expect to see from ultracentrifugation of these two substances? the sample loaded as a narrow band at the top of the tube (A) sample CENTRIFUGATION stabilizing sucrose gradient tube heterogeneous aggregates would sediment to produce a diffuse smear hemoglobin protein sediments as a single bandarrow_forwardWhen sequencing proteins, one tries to generate overlapping peptides by using cleavages at specific sites. Which of the following statements about cleavages caused by particular chemicals or enzymes are true? Cyanogen bromide cleaves at the carboxyl side of threonine. Chymotrypsin cleaves at the carboxyl side of aromatic amino acids. Trypsin cleaves at the carboxyl side of Lys and Arg. Chymotrypsin cleaves at the carboxyl side of aspartate and glutamate.arrow_forwardSubject: chemistryarrow_forward
- Suppose that there is a protein consisting of two polypeptide chains with the given sequences in the picture. Before performing Edman sequencing method, it is required to identify the protein's amino acid composition first. Explain why it is required to do the said step first before doing Edman sequencing method.arrow_forwardDescribe the common strategy (steps) for protein sequencing, starting with a biological sample containing many cell and biochemical substances. How prevalent are disulfide links in proteins? Why do the disulfide links need to be broken prior to sequencing? How can they be chemically broken?arrow_forwardDescribe the typical principles used to identify topogenic sequences within proteins and how these principles can be used to develop computer algorithms. How does the identification of topogenic sequences lead to prediction of the membrane arrangement of a multipass protein?arrow_forward
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