Biology: Life on Earth with Physiology (11th Edition)
11th Edition
ISBN: 9780133923001
Author: Gerald Audesirk, Teresa Audesirk, Bruce E. Byers
Publisher: PEARSON
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Question
Chapter 14.3, Problem 1CYL
Summary Introduction
To explain:
The role of polymerase chain reaction, gel electrophoresis, and DNA probes, and how they operate.
Introduction:
Polymerase chain reaction can be used to make several copies of selected pieces of DNA; it is developed by Kary Mullis in 1986. Gel electrophoresis represents a biological technique by which a mixture of DNA fragments can be separated under the influence of the electric field. DNA probe is used to detect a specific segment of DNA by using hybridization technique.
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What are DNA probes and for what are they used?
b) Describe how DNA is digested by different restriction enzymes
c) Describe how gel electrophoresis is used to estimate the size of DNA fragments.
What are the advantages of doing DNA sequencing?
Chapter 14 Solutions
Biology: Life on Earth with Physiology (11th Edition)
Ch. 14.1 - define biotechnology?Ch. 14.1 - Prob. 2CYLCh. 14.2 - describe natural processes that recombine DNA,...Ch. 14.3 - Guilty or Innocent? When biological evidence was...Ch. 14.3 - For any single person, a given STR always has...Ch. 14.3 - Prob. 2CSCCh. 14.3 - There are many other applications in which DNA...Ch. 14.3 - Prob. 1CYLCh. 14.3 - Prob. 2CYLCh. 14.3 - Prob. 3CYL
Ch. 14.4 - Restriction enzymes are isolated from bacteria....Ch. 14.4 - explain how genes are inserted into a plasmid, and...Ch. 14.4 - Prob. 2CYLCh. 14.5 - Prob. 1HYEWCh. 14.5 - describe the advantages of genetically modified...Ch. 14.5 - list some examples of how GM animals might be...Ch. 14.5 - Prob. 3CYLCh. 14.6 - Prob. 1CYLCh. 14.6 - explain how knowledge of the genomes of humans and...Ch. 14.7 - Prob. 1TCCh. 14.7 - explain how biotechnology is used to diagnose both...Ch. 14.7 - describe the procedures and advantages of gene...Ch. 14.8 - Genetic engineering is used both in food crops and...Ch. 14.8 - explain why people might be opposed to the use of...Ch. 14.8 - envision circumstances in which it would be...Ch. 14.8 - Prob. 1CSRCh. 14.8 - Prob. 2CTCh. 14 - Prob. 1MCCh. 14 - Prob. 2MCCh. 14 - Prob. 3MCCh. 14 - A restriction enzyme a. cuts DNA at a specific...Ch. 14 - Prob. 5MCCh. 14 - Prob. 1FIBCh. 14 - _________is the process whereby bacteria pick up...Ch. 14 - The _______ is a technique tor multiplying DNA in...Ch. 14 - Matching DNA samples in forensics uses a specific...Ch. 14 - Prob. 5FIBCh. 14 - Prob. 1RQCh. 14 - Prob. 2RQCh. 14 - Prob. 3RQCh. 14 - Prob. 4RQCh. 14 - Prob. 5RQCh. 14 - How does gel electrophoresis separate pieces of...Ch. 14 - Prob. 7RQCh. 14 - Prob. 8RQCh. 14 - Prob. 9RQCh. 14 - Prob. 10RQCh. 14 - As you may know, many Insects have evolved...Ch. 14 - Prob. 2AC
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- Explain how electrophoresis separates DNA strands. How is a DNA fingerprinting test interpreted?arrow_forwardHow is DNA amplified in a polymerase chain reaction(PCR) procedure?arrow_forwardBriefly explain how the polymerase chain reaction is used to amplify a specific DNA sequence. What are some of the limitations of PCR?arrow_forward
- Explain the importance of how Polymerase Chain Reaction, Gel Electrophoresis and Restrictions Enzymes can be used in the real world.arrow_forwardGel electrophoresis can be used for all of the following purposes except A) Determining the function of a gene product B Separating DNA (c) Assisting with constructing a restriction map (D) Determining the size of a DNA fragmentarrow_forwardWhat are the disadvantages of doing DNA sequencing?arrow_forward
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