Biology: Life on Earth with Physiology (11th Edition)
11th Edition
ISBN: 9780133923001
Author: Gerald Audesirk, Teresa Audesirk, Bruce E. Byers
Publisher: PEARSON
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Chapter 14, Problem 3RQ
Summary Introduction
To describe:
The term restriction enzymes.
Introduction:
Restriction enzymes are also known as molecular scissors. These cut the DNA (deoxyribonucleic acid) fragments at recognition sites and produce restriction fragments. Restriction enzymes or endonucleases were discovered by Arber, Nathans, and Smith. Some of the examples of restriction enzymes are Hind III (Haemophilus influenza, strain Rd, discovery order-III), BamHI (Bacillus amyloliquefaciens, strain H, discovery order-I), Hpa I (Haemophilus parainfluenzae, discovery order-I), and Sma I (Serratia marcescens, discovery order-I).
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Chapter 14 Solutions
Biology: Life on Earth with Physiology (11th Edition)
Ch. 14.1 - define biotechnology?Ch. 14.1 - Prob. 2CYLCh. 14.2 - describe natural processes that recombine DNA,...Ch. 14.3 - Guilty or Innocent? When biological evidence was...Ch. 14.3 - For any single person, a given STR always has...Ch. 14.3 - Prob. 2CSCCh. 14.3 - There are many other applications in which DNA...Ch. 14.3 - Prob. 1CYLCh. 14.3 - Prob. 2CYLCh. 14.3 - Prob. 3CYL
Ch. 14.4 - Restriction enzymes are isolated from bacteria....Ch. 14.4 - explain how genes are inserted into a plasmid, and...Ch. 14.4 - Prob. 2CYLCh. 14.5 - Prob. 1HYEWCh. 14.5 - describe the advantages of genetically modified...Ch. 14.5 - list some examples of how GM animals might be...Ch. 14.5 - Prob. 3CYLCh. 14.6 - Prob. 1CYLCh. 14.6 - explain how knowledge of the genomes of humans and...Ch. 14.7 - Prob. 1TCCh. 14.7 - explain how biotechnology is used to diagnose both...Ch. 14.7 - describe the procedures and advantages of gene...Ch. 14.8 - Genetic engineering is used both in food crops and...Ch. 14.8 - explain why people might be opposed to the use of...Ch. 14.8 - envision circumstances in which it would be...Ch. 14.8 - Prob. 1CSRCh. 14.8 - Prob. 2CTCh. 14 - Prob. 1MCCh. 14 - Prob. 2MCCh. 14 - Prob. 3MCCh. 14 - A restriction enzyme a. cuts DNA at a specific...Ch. 14 - Prob. 5MCCh. 14 - Prob. 1FIBCh. 14 - _________is the process whereby bacteria pick up...Ch. 14 - The _______ is a technique tor multiplying DNA in...Ch. 14 - Matching DNA samples in forensics uses a specific...Ch. 14 - Prob. 5FIBCh. 14 - Prob. 1RQCh. 14 - Prob. 2RQCh. 14 - Prob. 3RQCh. 14 - Prob. 4RQCh. 14 - Prob. 5RQCh. 14 - How does gel electrophoresis separate pieces of...Ch. 14 - Prob. 7RQCh. 14 - Prob. 8RQCh. 14 - Prob. 9RQCh. 14 - Prob. 10RQCh. 14 - As you may know, many Insects have evolved...Ch. 14 - Prob. 2AC
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- What are palindromes, and how are they related to restriction endonucleases? Why are they useful for inserting one piece of DNA into another?arrow_forwardBesides their role in genetic engineering, what else do plasmids can plasmids be used for? How are restriction enzymes used in genetic engineering?arrow_forwardWhat are the three types of DNA ends that can be generated after cutting DNA with restriction enzymes? What reaction is catalyzed by DNA ligase?arrow_forward
- In making recombinant DNA, what is the benefit of using a restriction enzyme that cuts DNA in a staggered fashion?arrow_forwardDEFINE THE FOLLOWING: 1) restriction enzyme 2) plasmid 3) recombinant DNAarrow_forwardWhich of the following is necessary for a PCR reaction to proceed? a) the sequence of the ends of the DNA to be amplified must be known. b) the sequence of restriction endonuclease recognition sites in the DNA to be amplified and in the plasmid, where the amplified DNA fragment will be cloned must be known. c) The complete sequence of the DNA to be amplified must be known. d) The sequence of restriction endonuclease recognition sites in the DNA to be amplified must be knownarrow_forward
- Why do restriction endonucleases not hydrolyze DNA from the organism that produces it?arrow_forwardb) Describe how DNA is digested by different restriction enzymes c) Describe how gel electrophoresis is used to estimate the size of DNA fragments.arrow_forwardWhen making recombinant DNA, why must you use the same restriction enzyme to cut the gene of interest and the plasmid?arrow_forward
- What roles do restriction enzymes, vectors, and host cells play in recombinant DNA studies? What role does DNA ligase perform in a DNA cloning experiment? How does the action of DNA ligase differ from the function of restriction enzymes?arrow_forwardWhat is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into bacterium?I) Transform bacteria with recombinant DNA molecule.II) Cut the plasmid DNA using restriction enzymes.III) Extract plasmid DNA from bacterial cells.IV) Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.V) Use ligase to seal plasmid DNA to non-plasmid DNA A. III, II, IV, V, I B. I, II, IV, III, V C. III, IV, V, I, II D. II, III, V, IV, Iarrow_forwardFrom where do we get primers for sequencing DNA? A) they are synthesized by reverse transcriptase B) they are cut out of plasmids using restriction endonucleases C) DNA primase is added to the sequencing reaction and synthesizes the primers D) biotechnology companies synthesize them using organic chemistryarrow_forward
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