Biology: Life on Earth with Physiology (11th Edition)
Biology: Life on Earth with Physiology (11th Edition)
11th Edition
ISBN: 9780133923001
Author: Gerald Audesirk, Teresa Audesirk, Bruce E. Byers
Publisher: PEARSON
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Chapter 14.3, Problem 1CSC

Guilty or Innocent?

When biological evidence was found in one of the Haynesworth case files in 2009, the sample was 25 years old. Fortunately DNA doesn't degrade very fast. Forensic lab technicians amplified the DNA with PCR so that they had enough material to analyze. How did the lab use STRs to determine that the semen profile matched Davis, and not Haynesworth?

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The following shows the results of DNA profiling from a rape case. The evidence sample (S) consists of a mixture of DNA from the victim (V) and the DNA of the rapist(s). DNA is taken from four likely suspects (who were present at the same party as the victim on the night of the crime), Alan (A), Brad (B), Charle (C), and Don (D). The results of typing for two different polymorphic loci are shown. 1)Which person is homozygous for locus 1? A. Alan B. Brad C. Charlie D. Don E. Victim
Which samples contain the blood of the father of the child?
1) A forensic scientist is required to collect blood samples from the crime scene and analyze it to help the investigators understand who participated to the murder. i) As a first step, the scientist needs to extract DNA. Indicate why red blood cells are not a good target for the analysis, and list at least two chemicals/compounds that are required to extract the DNA, explaining why they are used. ii) The sample has been collected in a small amount, so that (s)he decides to use PCR to analyze it. Describe why PCR is better suited to address small samples with respect to Southern blotting and how is selectivity addressed with this technique.

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Biology: Life on Earth with Physiology (11th Edition)

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