Prescott's Microbiology
Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Chapter 14, Problem 2CHI

Describe the phenotype of the following E. coli mutants when grown in two different media: glucose only and lactose only. Explain the reasoning behind your answer.

  1. a. A strain with a mutation in the gene encoding the lac repressor; the mutant repressor cannot bind allolactose.
  2. b. A strain with a mutation in the gene encoding CAP; the mutant form of CAP binds but cannot release cAMP.
  3. c. A strain in which the Shine-Dalgarno sequence has been deleted from the gene encoding adenyl cyclase.
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