Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Chapter 14.5, Problem 1.5RIA
Summary Introduction
A sensory receptor involved in the transduction of chemical signal into an action potential is referred to as a chemoreceptor. They are located on the cell membrane and are capable of detecting specific
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Increasing the saturation of the ammonium sulfate is a prerequisite in isolating a target protein that is rich in Cys and Tyr residues. Which of the following techniques should be considered in accurately quantifying the isolated protein?I. Running the isolated protein in a dialysis or GFC set up.II. Using Biuret or BCA assay as the colorimetric quantitation method.III. Using Bradford or Lowry assay as the colorimetric quantitation method.A. I onlyB. II onlyC. I and IIID. I, II and III. Bradford Assay is most suitable to use when the extraction buffer is below the target protein’s pI. This is so because the protein would be morea. Positively charged allowing the CBB G-250 dye to bind via its sulfonate groups.b. Negatively charged allowing the CBB G-250 dye to bind via its sulfonate groups.c. Neutrally charged allowing the CBB G-250 dye to bind via its sulfonate groups.d. Zwitterionic allowing the CBB G-250 dye to bind via its sulfonate groups.
Plz choose all correct option and Do explain.
Which of the following descriptions about tandem affinity purification is not correct?
a.The interaction between calmodulin-binding peptides and calmodulin beads can be broken by adding EDT
b.In the first round of elution, TEV protease cleaves the cleavage site on the TAP-fused target protein.
c.It enables the purification of protein complexes composed of multiple protein components.
d.It is possible to perform two rounds of purification using a single TAP tag.
e.The protein A tag binds to beads that have lgG attached to them.
Chapter 14 Solutions
Prescott's Microbiology
Ch. 14.2 - MICRO INQUIRY In what way is on inducer molecule...Ch. 14.2 - MICRO INQUIRY Is allolactose a corepressor or...Ch. 14.2 - Retrieve, Infer, Apply Many genes and operons are...Ch. 14.2 - Retrieve, Infer, Apply What are induction and...Ch. 14.2 - Retrieve, Infer, Apply Using figure 14.4 as a...Ch. 14.3 - MICRO INQUIRY How does this attenuation respond to...Ch. 14.4 - MICRO INQUIRY How does inhibition of translation...Ch. 14.4 - Prob. 1RIACh. 14.4 - Retrieve, Infer, Apply Describe how attenuation...Ch. 14.4 - Retrieve, Infer, Apply What are translational...
Ch. 14.4 - Retrieve, Infer, Apply How are translational...Ch. 14.4 - Prob. 5RIACh. 14.5 - MICRO INQUIRY Relative to each promoter, where...Ch. 14.5 - MICRO INQUIRY For what other compounds would you...Ch. 14.5 - Prob. 3MICh. 14.5 - MICRO INQUIRY Why does V. harveyi make three...Ch. 14.5 - Prob. 5MICh. 14.5 - Prob. 6MICh. 14.5 - Prob. 1.1RIACh. 14.5 - Retrieve, Infer, Apply What is diauxic growth?...Ch. 14.5 - Retrieve, Infer, Apply Describe the events that...Ch. 14.5 - Retrieve, Infer, Apply E. coli has two phosphate...Ch. 14.5 - Prob. 1.5RIACh. 14.5 - Prob. 1.6RIACh. 14.5 - Prob. 2.1RIACh. 14.5 - Retrieve, Infer, Apply Why might bacteria use...Ch. 14.5 - Prob. 2.3RIACh. 14.5 - Prob. 2.4RIACh. 14 - Attenuation affects anabolic pathways, whereas...Ch. 14 - Describe the phenotype of the following E. coli...Ch. 14 - Prob. 3CHICh. 14 - What would be the phenotype of a B. subtilis...Ch. 14 - Propose a mechanism by which a cell might sense...Ch. 14 - Neisseria meningitidis, commonly called...
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