Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 14.5, Problem 1MI
MICRO INQUIRY
Relative to each promoter, where would you predict phosphorylated OmpR would bind the ompC and ompF genes?
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E30. An electrophoretic mobility shift assay can be used to study the
binding of proteins to a segment of DNA. In the experiment shown
here, an EMSA was used to examine the requirements for the bind-
ing of RNA polymerase II (from eukaryotic cells) to the promoter
of a protein-encoding gene. The assembly of general transcription
factors and RNA polymerase II at the core promoter is described
in Chapter 12 (Figure 12.14). In this experiment, the segment of
DNA containing a promoter sequence was 1100 bp in length. The
fragment was mixed with various combinations of proteins and
then subjected to an EMSA.
Lane 1: No proteins added
Lane 2: TFID
Lane 3: TFIIB
Lane 4: RNA polymerase I|
Lane 5: TFID + TFIIB
Lane 6: TFID + RNA
1 2
4 5 6
polymerase II|
Lane 7: TFIID +
TFIIB + RNA
polymerase I|
1100 bp
Explain which proteins (TFIID, TFIIB, or RNA polymerase II) are
able to bind to this DNA fragment by themselves. Which transcrip-
tion factors (i.e., TFIID or TFIIB) are needed for the binding of…
Pls help ASAP
Chapter 14 Solutions
Prescott's Microbiology
Ch. 14.2 - MICRO INQUIRY In what way is on inducer molecule...Ch. 14.2 - MICRO INQUIRY Is allolactose a corepressor or...Ch. 14.2 - Retrieve, Infer, Apply Many genes and operons are...Ch. 14.2 - Retrieve, Infer, Apply What are induction and...Ch. 14.2 - Retrieve, Infer, Apply Using figure 14.4 as a...Ch. 14.3 - MICRO INQUIRY How does this attenuation respond to...Ch. 14.4 - MICRO INQUIRY How does inhibition of translation...Ch. 14.4 - Prob. 1RIACh. 14.4 - Retrieve, Infer, Apply Describe how attenuation...Ch. 14.4 - Retrieve, Infer, Apply What are translational...
Ch. 14.4 - Retrieve, Infer, Apply How are translational...Ch. 14.4 - Prob. 5RIACh. 14.5 - MICRO INQUIRY Relative to each promoter, where...Ch. 14.5 - MICRO INQUIRY For what other compounds would you...Ch. 14.5 - Prob. 3MICh. 14.5 - MICRO INQUIRY Why does V. harveyi make three...Ch. 14.5 - Prob. 5MICh. 14.5 - Prob. 6MICh. 14.5 - Prob. 1.1RIACh. 14.5 - Retrieve, Infer, Apply What is diauxic growth?...Ch. 14.5 - Retrieve, Infer, Apply Describe the events that...Ch. 14.5 - Retrieve, Infer, Apply E. coli has two phosphate...Ch. 14.5 - Prob. 1.5RIACh. 14.5 - Prob. 1.6RIACh. 14.5 - Prob. 2.1RIACh. 14.5 - Retrieve, Infer, Apply Why might bacteria use...Ch. 14.5 - Prob. 2.3RIACh. 14.5 - Prob. 2.4RIACh. 14 - Attenuation affects anabolic pathways, whereas...Ch. 14 - Describe the phenotype of the following E. coli...Ch. 14 - Prob. 3CHICh. 14 - What would be the phenotype of a B. subtilis...Ch. 14 - Propose a mechanism by which a cell might sense...Ch. 14 - Neisseria meningitidis, commonly called...
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