Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Textbook Question
Chapter 14, Problem 13P
The CBF genes of Arabidopsis are induced by exposure of the plants to low temperature.
How would you examine the temporal and spatial patterns of expression after induction by low temperature?
Can you design a method that would reveal these changes in gene expression in a way that a farmer could recognize them by observing plants growing in the field?
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
Many plants increase in freezing tolerance upon exposure to low nonfreezing
temperatures. Rye, for instance, is killed by freezing at about -5°C, but after a period
of exposure to low nonfreezing temperature can survive freezing down to about
-30°C. When exposed to low temperatures, plants exhibit an increased expression
of certain cold-induced genes that function to stabilize cell membranes against
freeze-induced injury. The ability of an individual plant to develop freezing tolerance
is best described as an example of:
Genetic drift
OMutation
O Allopatric speciation
OAdaptation
Acclimation
Freeze-induced cellular dehydration
Many plants increase in freezing tolerance upon exposure to low nonfreezing
temperatures. Rye, for instance, is killed by freezing at about -5°C, but after a period
of exposure to low nonfreezing temperature can survive freezing down to about
-30°C. When exposed to low temperatures, plants exhibit an increased expression
of certain cold-induced genes that function to stabilize cell membranes against
freeze-induced injury. The ability of an individual plant to develop freezing tolerance
is best described as an example of:
O Acclimation
Allopatric speciation
O Mutation
Genetic drift
OAdaptation
Freeze-induced cellular dehydration
Many plants increase in freezing tolerance upon exposure to low nonfreezing
temperatures. Rye, for instance, is killed by freezing at about -5°C, but after a period
of exposure to low nonfreezing temperature can survive freezing down to about
-30°C. When exposed to low temperatures, plants exhibit an increased expression of
certain cold-induced genes that function to stabilize cell membranes against freeze-
induced injury. The ability of an individual plant to develop freezing tolerance is best
described as an example of:
Allopatric speciation
Freeze-induced cellular dehydration
Adaptation
Acclimation
O Mutation
Genetic drift
Chapter 14 Solutions
Genetic Analysis: An Integrated Approach (3rd Edition)
Ch. 14 - 14.1 What are the advantages and disadvantages of...Ch. 14 - Prob. 2PCh. 14 - Discuss the similarities and differences between...Ch. 14 - 14.5 What are the advantages and disadvantages of...Ch. 14 - 14.6 You have cloned the mouse ortholog (see...Ch. 14 - 14.7 Diagram the mechanism by which CRISPRCas...Ch. 14 - 14.8 Describe how CRISPRCas has been modified to...Ch. 14 - 14.9 Discuss the advantages (and possible...Ch. 14 - 14.10 Discuss the advantages (and possible...Ch. 14 - You have identifies a gene encoding the protein...
Ch. 14 - You have identified a recessive mutation that...Ch. 14 - 14.13 The CBF genes of Arabidopsis are induced by...Ch. 14 - 14.14 When the S. cerevisiae genome was sequenced,...Ch. 14 - 14.15 Translational fusions between a protein of...Ch. 14 - 14.16 In humans, Duchenne’s muscular dystrophy is...Ch. 14 - 14.17 How would you perform a genetic screen to...Ch. 14 - In enhancer trapping experiments, a minimal...Ch. 14 - 14.19 In Genetic Analysis, we designed a screen to...Ch. 14 - How would you design a genetic screen to find...Ch. 14 - 14.21 The eyes of Drosophila develop from imaginal...Ch. 14 - 14.22 Given your knowledge of the genetic tools...Ch. 14 - Mutations in the CFTR gene result in cystic...Ch. 14 - 14.24 How would you clone a gene that you have...Ch. 14 - 14.25 How would you conduct a screen to identify...Ch. 14 - In land plants, there is an alternation of...Ch. 14 - 14.27 The Drosophila evenskipped (eve) gene is...Ch. 14 - Prob. 28PCh. 14 - 14.29 As shown in Figure, mutations in the...Ch. 14 - How would you edit a specific nucleotide in a...Ch. 14 - Through a forward genetics screen in Arabidopsis...Ch. 14 - The CRISPR - Cas 9 complex directs the Cas 9...Ch. 14 - 14.33 Describe how enhancer screens can be used to...Ch. 14 - How might you use CRISPR - Cas 9 to create a large...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- You conduct an experiment to study the expression of the S protein through the tissues of your favorite plant (Arabidopsis thaliana). The morning of the experiment you inject in the leaves a messenger RNA that codes for the synthesis of a single protein made of two parts that are attached to each other: the functional S protein and a red fluorescent protein tag (RFP). You perform two cross sections of the same root, one section at the start of the experiment (time = Oh; corresponding to the time of injection) and one section in the afternoon (time = 8h). Through fluorescent microscopy you observe a change in the coloration inside the cells of the root's central tissues (as indicated by the arrows): from no coloration (time = Oh) to red (time = 8h). • The cells of the central tissues in the roots do not have nuclei or ribosomes. How can you explain this change of coloration? Please provide a cellular feature that can lead to this. • What is one advantage of using an RFP-tag in an mRNA?…arrow_forwardIn roses, the synthesis of red pigment is produced by two steps in a pathway. gene O magenta intermediate - gene P colorless intermediate- red pigment What would the phenotype be of a plant homozygous for a null mutation of gene P? What would the phenotype be of a plant homozygous for a null mutation of gene Q? What would the phenotype be of a plant homozygous for null mutations of genes P and Q? magenta red Match a genotype to each strain. colorless Strain P locus Q locus homozygous null mutation of gene P homozygous null mutation of gene Q homozygous null mutations of genes P and Q Answer Bank plp PIP What F2 ratio is expected from crossing a plant that is homozygous for a null mutation of gene P with a plant that is homozygous for a null mutation of gene Q? Assume independent assortment. 9 colorless : 4 magenta : 3 red 9 red : 4 colorless : 3 magenta O 9 red : 4 magenta : 3 colorlessarrow_forwardIn Arabidopsis, it is well-known that a pulse of full-spectrum light during the night (in an otherwise long night) will induce flowering. This suggests that plants measure the length of night, and not the length of day. If the pulse of light during the night was blue light instead of full spectrum light, what would be the flowering time response of a plant with a knockout in cry2 (relative to wild type in the same conditions)? Explain.arrow_forward
- Boll weevil is a serious pest of cotton crop. Effective control involves applications of chemical insecticides, increasing the cost of production and environmental pollution. The current genetically modified Bt crops have allowed great benefits to farmers but show activity limited to lepidopteran pests. This work reports on procedures adopted for integration and expression of a cry transgene conferring resistance to boll weevil and fall armyworm by using molecular tools. Four Brazilian cotton cultivars were microinjected with a minimal linear cassette generating 1248 putative lines. Complete gene integration was found in only one line (TO-34) containing one copy of crylla detected by Southern blot. Protein was expressed in high concentration at 45 davs after emergence (dae), decreasing by approximately 50% at 90 dae. Toxicity of the cry protein was demonstrated in feeding bioassays revealing 56.7% mortality to boll weevil fed buds and 88.1% mortality to fall armyworm fed leaves. A…arrow_forwardCan you solve all the parts to this question please a) What is the role of the LEAFY gene in floral development? (b) What is the phenotype of the leafy mutant? (c) Most of the ABC program genes are directly regulated by the transcription factor LEAFY (LFY). Still, LFY is expressed broadly throughout the floral meristem when the discrete A, B, and C domains are established. Discuss how this single broadly expressed transcription factor can generate distinct domains of ABC gene expression. Feel free to use diagrams.arrow_forwardIf Gus is present in the Arabidopsis Thaliana what is it indicating? I know its to show gene expression, but what is the importance of that? I hope this makes sensearrow_forward
- Trace the genetic steps in the development of a peanut plant that isresistant to insect larvae (table given). (Hint: You need to useAgrobacterium.)arrow_forwardAn agriculture lab is trying to improve a type of tomato so that it produces large fruits even under very hot, dry conditions. To do this, they must first identify the genes responsible for the tomato's response to temperature and moisture. Which experimental approach would be most helpful in doing this?arrow_forwardThe floral homeotic genes of Arabidopsis belong to the MADS-box gene family, while in Drosophila, homeotic genes belong to the homeobox gene family. In both Arabidopsis and Drosophila, members of the Polycomb gene family control expression of these divergent homeotic genes. How do Polycomb genes control expression of two very different sets of homeotic genes?arrow_forward
- How do further studies on the ACO5 gene benefit gene expression for Arabidopsis thaliana in future work?arrow_forwardIn roses, the synthesis of red pigment is by two steps ina pathway, as follows:colorless intermediate gene Pmagenta intermediate red pigment gene Qa. What would the phenotype be of a plant homozygousfor a null mutation of gene P?b. What would the phenotype be of a plant homozygousfor a null mutation of gene Q?c. What would the phenotype be of a plant homozygousfor null mutations of genes P and Q?d. Write the genotypes of the three strains in parts a, b,and c.e. What F2 ratio is expected from crossing plants fromparts a and b? (Assume independent assortment.)arrow_forwardA -------| B-------> Violet A linear switch-regulation pathway of the type shown here controls flower color in a certain species. The wildtype color is violet, but mutants can be either white or purple. Gene products A and B act in the pathway at the positions and in the manner shown (A is inhibitory, B is intensifying). A and B are the products of genes A and B, respectively. What are the expected phenotypes of: (a) A loss-of-function mutation in A? (b) A gain-of-function mutation in A? (c) A loss-of-function mutation in B? (d) A gain-of-function mutation in B?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
QCE Biology: Introduction to Gene Expression; Author: Atomi;https://www.youtube.com/watch?v=a7hydUtCIJk;License: Standard YouTube License, CC-BY