Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Textbook Question
Chapter 14, Problem 10P
Discuss the advantages (and possible disadvantages) of the different mutagens in Table 14.1.
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Mutagenesis is a technique in which genetic information of an organism is altered in a stable
manner resulting in a mutation. It may occur spontaneously in nature of as a result of
exposure to mutagens. It can also be achieved experimentally using optimized laboratory
procedures.
(i)
(ii)
What is site directed mutagenesis (SDM)?
Explain how SDM can assist in the integration of a His-tag at the end of your gene of
interest.
what is thermal stability and explain how the thermal stability of named proteins were improved using site directed mutagenesis.
Based on the following wild type DNA sequence, indicate if each of the
mutations should be classified as : insertion, deletion, missense, nonsense,
silent (Use the provided Genetic Code table and remember you have been
given DNA sequence).
Wild Type: AUGAUUCUUAAAAGU
Mutant 1: AUGAUUCUUUAAAGU
Mutant 2: AUGAUUCUUGAAAGU
Mutant 3: AUGAUCCUUAAAAGU
Mutant 4: AUGAUCCUAAAAGU
Mutant 5: AUGAUCCUUAAACAGU
Socond letter
Key:
Ala = Alanine (A)
Arg Arginine (R)
Asn =
UUU
}
UAU Tyr
UGU
UGC Cys
UGA STOP
UGG Trp
UCU
UCC
UUC
Phe
Ser
Asparagine (N)
Asp = Aspartate (D)
Cys Cysteine (C)
Gin = Glutamine (Q)
Glu = Glutamate (E)
Gly = Glycine (G)
His = Histidine (H)
le = Isoleucine (1)
Leucine (L)
Lys Lysine (K)
Met = Methionine (M)
Phe = Phenylalanine (F)
Pro Proline (P)
Ser = Serine (S)
Thr Threonine (T)
Trp Tryptophan (W)
Tyr Tyrosine (Y)
- Valine (V)
UCA
UCG
UAA STOP
UAG STOP
UUA
Leu
UUG S
CCU
CC
CGU
CUU
CUC
His
CGC
Arg
Leu
Pro
CAA Gin
CGA
CCA
CCG
CUA
CUG
CGG
Leu =
AGU
AUU
AUC } lle
AUA
ACU
ACC
ACA
Ser
AAC…
Chapter 14 Solutions
Genetic Analysis: An Integrated Approach (3rd Edition)
Ch. 14 - 14.1 What are the advantages and disadvantages of...Ch. 14 - Prob. 2PCh. 14 - Discuss the similarities and differences between...Ch. 14 - 14.5 What are the advantages and disadvantages of...Ch. 14 - 14.6 You have cloned the mouse ortholog (see...Ch. 14 - 14.7 Diagram the mechanism by which CRISPRCas...Ch. 14 - 14.8 Describe how CRISPRCas has been modified to...Ch. 14 - 14.9 Discuss the advantages (and possible...Ch. 14 - 14.10 Discuss the advantages (and possible...Ch. 14 - You have identifies a gene encoding the protein...
Ch. 14 - You have identified a recessive mutation that...Ch. 14 - 14.13 The CBF genes of Arabidopsis are induced by...Ch. 14 - 14.14 When the S. cerevisiae genome was sequenced,...Ch. 14 - 14.15 Translational fusions between a protein of...Ch. 14 - 14.16 In humans, Duchenne’s muscular dystrophy is...Ch. 14 - 14.17 How would you perform a genetic screen to...Ch. 14 - In enhancer trapping experiments, a minimal...Ch. 14 - 14.19 In Genetic Analysis, we designed a screen to...Ch. 14 - How would you design a genetic screen to find...Ch. 14 - 14.21 The eyes of Drosophila develop from imaginal...Ch. 14 - 14.22 Given your knowledge of the genetic tools...Ch. 14 - Mutations in the CFTR gene result in cystic...Ch. 14 - 14.24 How would you clone a gene that you have...Ch. 14 - 14.25 How would you conduct a screen to identify...Ch. 14 - In land plants, there is an alternation of...Ch. 14 - 14.27 The Drosophila evenskipped (eve) gene is...Ch. 14 - Prob. 28PCh. 14 - 14.29 As shown in Figure, mutations in the...Ch. 14 - How would you edit a specific nucleotide in a...Ch. 14 - Through a forward genetics screen in Arabidopsis...Ch. 14 - The CRISPR - Cas 9 complex directs the Cas 9...Ch. 14 - 14.33 Describe how enhancer screens can be used to...Ch. 14 - How might you use CRISPR - Cas 9 to create a large...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- List three possible uses of site-directed mutagenesisarrow_forwardCan someone tell me why the answer is that the spill contained transition inducing mutagens?arrow_forwardTelomerase activity has been found to be 10 to 20 times more active in cancer cells than in normal somatic cells. What is the significance of this circumstance?arrow_forward
- I have complete the table that required. Indicate which mutations were success why some were not from the size about. indicate at what stage any of the mutagenesis failed.arrow_forwardYou plan to clone exon 11 of the HEXA gene (Section C) into the multiple cloning site (MCS) of the plasmid vector pUC19 illustrated below. You PCR amplify only the 184 bp DNA region representing exon 11 of HEXA from human DNA as a blunt-ended dsDNA fragment, and purify the amplicon. Next you digest the vector pUC19 with the restriction enzyme (RE) Smal to obtain linear plasmid DNA. You mix the PCR product and linear plasmid, add some DNA ligase enzyme in an appropriate buffer, and incubate overnight at 16°C. The ligation mixture is used to transform competent E. coli cells, which are subsequently streaked out onto agar plates containing ampicillin, X-gal and IPTG. HEXA exon 11 sequence: attcagccagacacaatcatacaggtgtggcgagaggatattccagtgaactatatgaaggagctggaactggtc accaaggccggcttccgggcccttctctctgccccctggtacctgaaccgtatatcctatggccctgactggaag gatttctacatagtggaacccctggcatttgaag PUC 19 plasmid map: 2686 1 Amp 0 lacZ EcoRI (390) Smal (410) BamHI (420) MCS Kpnl (430) LacR binding site Plac Pstl…arrow_forwardYou are working with a newly discovered mutagen, and you wish to determine the base change that it introduces into DNA. Thus far, you have determined that the mutagen chemically alters a single base in such a way that its base-pairing properties are altered permanently. To determine the specificity of the alteration, you examine the amino acid changes that take place after mutagenesis. A sample of what you find is shown here:Original: Gln–His–Ile–Glu–LysMutant: Gln–His–Met–Glu–LysOriginal: Ala–Val–Asn–ArgMutant: Ala–Val–Ser–ArgOriginal: Arg–Ser–LeuMutant: Arg–Ser–Leu–Trp–Lys–Thr–Phearrow_forward
- Besides the examples listed in Table 24.3, list five types ofshort sequences that a geneticist might want to locate withina DNA sequence.arrow_forwardIn the Ames test shown in Figure 16-17, what is the reason for adding the liver extract to each sample?arrow_forwardContrast and compare the mutagenic effects of deaminating agents, alkylating agents, and base analogsarrow_forward
- You are working with a newly discovered mutagen, andyou wish to determine the base change that it introduces into DNA. Thus far, you have determined that themutagen chemically alters a single base in such a waythat its base-pairing properties are altered permanently.To determine the specificity of the alteration, you examine the amino acid changes that take place after mutagenesis. A sample of what you find is shown here:Original: Gln–His–Ile–Glu–LysMutant: Gln–His–Met–Glu–LysOriginal: Ala–Val–Asn–ArgMutant: Ala–Val–Ser–ArgOriginal: Arg–Ser–LeuMutant: Arg–Ser–Leu–Trp–Lys–Thr–PheWhat is the base-change specificity of the mutagen?arrow_forwardA chemist synthesizes four new chemical compounds in the laboratory and names them PFI1, PFI2, PFI3, and PFI4. He gives the PFI compounds to a geneticist friend and asks her to determine their mutagenic potential. The geneticist finds that all four are highly mutagenic. She also tests the capacity of mutations produced by the PFI compounds to be reversed by other known mutagens and obtains the following results. What conclusions can you make about the nature of the mutations produced by these compounds? Reversed by Mutations, Nitrous, Aminopurine acid Acridine orange 2- produced by Hydroxylamine PFI1 Yes Yes Some No PFI2 No No No No PFI3 Yes Yes No No PFI4 No No No Yesarrow_forwardWhat is linker scanning mutagenesis?arrow_forward
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