Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 14, Problem 30P
How would you edit a specific
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Students have asked these similar questions
Consider the following DNA sequence:
-T
--
--
If RNA primase used this section of DNA to make a primer, what would be the
correct sequence of base pairs (from top to bottom)?
T-A-C-C-G-T-T
OT-U-C-C-G-U-U
OA-T-G-G-C-A-A
U-A-C-C-G-U-U
If I take random biological sequences from a data bank, I see even more sequence similarity. Why ?
I have built an urn model for a DNA sequence. I have taken the letters from a sequence 1946 nucleotides long. The urn contains 259 Adenines, 699 Cytosines, 401 Thymines and 587 Guanines
How many start codons will I find on average in the entire sequence when I draw all of the bases from the urn without replacement?
Please I'm not too sure how to tackle this.
Chapter 14 Solutions
Genetic Analysis: An Integrated Approach (3rd Edition)
Ch. 14 - 14.1 What are the advantages and disadvantages of...Ch. 14 - Prob. 2PCh. 14 - Discuss the similarities and differences between...Ch. 14 - 14.5 What are the advantages and disadvantages of...Ch. 14 - 14.6 You have cloned the mouse ortholog (see...Ch. 14 - 14.7 Diagram the mechanism by which CRISPRCas...Ch. 14 - 14.8 Describe how CRISPRCas has been modified to...Ch. 14 - 14.9 Discuss the advantages (and possible...Ch. 14 - 14.10 Discuss the advantages (and possible...Ch. 14 - You have identifies a gene encoding the protein...
Ch. 14 - You have identified a recessive mutation that...Ch. 14 - 14.13 The CBF genes of Arabidopsis are induced by...Ch. 14 - 14.14 When the S. cerevisiae genome was sequenced,...Ch. 14 - 14.15 Translational fusions between a protein of...Ch. 14 - 14.16 In humans, Duchenne’s muscular dystrophy is...Ch. 14 - 14.17 How would you perform a genetic screen to...Ch. 14 - In enhancer trapping experiments, a minimal...Ch. 14 - 14.19 In Genetic Analysis, we designed a screen to...Ch. 14 - How would you design a genetic screen to find...Ch. 14 - 14.21 The eyes of Drosophila develop from imaginal...Ch. 14 - 14.22 Given your knowledge of the genetic tools...Ch. 14 - Mutations in the CFTR gene result in cystic...Ch. 14 - 14.24 How would you clone a gene that you have...Ch. 14 - 14.25 How would you conduct a screen to identify...Ch. 14 - In land plants, there is an alternation of...Ch. 14 - 14.27 The Drosophila evenskipped (eve) gene is...Ch. 14 - Prob. 28PCh. 14 - 14.29 As shown in Figure, mutations in the...Ch. 14 - How would you edit a specific nucleotide in a...Ch. 14 - Through a forward genetics screen in Arabidopsis...Ch. 14 - The CRISPR - Cas 9 complex directs the Cas 9...Ch. 14 - 14.33 Describe how enhancer screens can be used to...Ch. 14 - How might you use CRISPR - Cas 9 to create a large...
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- look at the code: import random # Define the DNA nucleotidesnucleotides = ["A", "T", "G", "C"] # Define the codon-to-amino acid dictionarycodon_table = { "AUG": "M", # Start codon "UUU": "F", "UUC": "F", "UUA": "L", "UUG": "L", "UCU": "S", "UCC": "S", "UCA": "S", "UCG": "S", "UAU": "Y", "UAC": "Y", "UAA": "*", # Stop codon "UAG": "*", # Stop codon "UGU": "C", "UGC": "C", "UGA": "*", # Stop codon "UGG": "W", "CUU": "L", "CUC": "L", "CUA": "L", "CUG": "L", "CCU": "P", "CCC": "P", "CCA": "P", "CCG": "P", "CAU": "H", "CAC": "H", "CAA": "Q", "CAG": "Q", "CGU": "R", "CGC": "R", "CGA": "R", "CGG": "R", "AUU": "I", "AUC": "I", "AUA": "I", "AUC": "I", "ACU": "T", "ACC": "T", "ACA": "T", "ACG": "T", "AAU": "N", "AAC": "N", "AAA": "K", "AAG": "K", "AGU": "S", "AGC": "S", "AGA": "R", "AGG": "R", "GUU": "V", "GUC": "V", "GUA": "V",…arrow_forwardDoes a sequence contain a gene?arrow_forwardThe genetic code uses three bases to encode one amino acid. Why can't the code use only two bases to encode each amino acid?arrow_forward
- Translate this nucleotide sequence into an amino acid sequence. Gene Sequence (5'-to-3'):…arrow_forwardIf you worked backward, starting with the amino acid sequence of the protein, would you obtain the same DNA nucleotide sequence? Why or why not?arrow_forwardUse the genetic code table to determine the amino acid sequence of the given message strand of DNA below, from N to C terminal. All introns were removed in this sequence for simplicity. Write the amino acids in their 3-letter abbreviation and separate with a dash.arrow_forward
- If a bacterial protein is 3,300 amino acids long, how many nucleotidepairs long is the gene sequence that codes for it?arrow_forwardTranscribe the following DNA sequence. Then translate the resulting mRNA transcript. GGACTACGTTCAAAAGCCATGGATTCGGTA Transcription: Translation: What would be the result of the following mutations in the DNA sequence above? How would the polypeptide change? How would you characterize this mutation? (Nucleotides are numbered from left to right.) a) nucleotide number 16 changes from a G to an A b) nucleotide number 12 changes from an A to a T c) nucleotide number 8 changes from a G to an A d) an insertion of a C between nucleotides 14 and 15.arrow_forward6a) Transcribe the following DNA sequence into codons. TACGCGACATTACATGAATCGTTTGGAGATTAGCCCTATTTCTCTAAGAACACGACTb) Excise(cut out) codons numbered 5, 6, and 7. Leave the remaining codons. c) Now translate the sequence . d) Explain how many amino acids are now in your polypeptide? e) What would happen to your polypeptide if either of your cysteine amino acids near the start or end of thepolypeptide were translated incorrectly. f) Based on your final polypeptide can you make the original DNA strand by doing reverse translation andtranscription? g) Explain if your polypeptide similar to your template strand or the complementary strand?arrow_forward
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