Chapter 14, Problem 14P

When the S. cerevisiae genome was sequenced, only about $40\%$ of its predicted genes had been previously identified in forward genetic screens. This left about $60\%$ of predicted genes with no known function, leading some to dub the genes fun (function unknown) genes.

As an approach to understanding the function of a certain fun gene, you wish to create a loss $\text{-}$ of $\text{-}$ function allele. How will you accomplish this?

You wish to know the physical location of the encoded protein product. How will you obtain such information?