Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 14, Problem 12P
You have identified a recessive mutation that alters bristle patterning in Drosophila and have used recombinant DNA technology to identify genomic clone that you believe harbors the gene. How would you demonstrate that your gene is on the genomic clone ?
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Chapter 14 Solutions
Genetic Analysis: An Integrated Approach (3rd Edition)
Ch. 14 - 14.1 What are the advantages and disadvantages of...Ch. 14 - Prob. 2PCh. 14 - Discuss the similarities and differences between...Ch. 14 - 14.5 What are the advantages and disadvantages of...Ch. 14 - 14.6 You have cloned the mouse ortholog (see...Ch. 14 - 14.7 Diagram the mechanism by which CRISPRCas...Ch. 14 - 14.8 Describe how CRISPRCas has been modified to...Ch. 14 - 14.9 Discuss the advantages (and possible...Ch. 14 - 14.10 Discuss the advantages (and possible...Ch. 14 - You have identifies a gene encoding the protein...
Ch. 14 - You have identified a recessive mutation that...Ch. 14 - 14.13 The CBF genes of Arabidopsis are induced by...Ch. 14 - 14.14 When the S. cerevisiae genome was sequenced,...Ch. 14 - 14.15 Translational fusions between a protein of...Ch. 14 - 14.16 In humans, Duchenne’s muscular dystrophy is...Ch. 14 - 14.17 How would you perform a genetic screen to...Ch. 14 - In enhancer trapping experiments, a minimal...Ch. 14 - 14.19 In Genetic Analysis, we designed a screen to...Ch. 14 - How would you design a genetic screen to find...Ch. 14 - 14.21 The eyes of Drosophila develop from imaginal...Ch. 14 - 14.22 Given your knowledge of the genetic tools...Ch. 14 - Mutations in the CFTR gene result in cystic...Ch. 14 - 14.24 How would you clone a gene that you have...Ch. 14 - 14.25 How would you conduct a screen to identify...Ch. 14 - In land plants, there is an alternation of...Ch. 14 - 14.27 The Drosophila evenskipped (eve) gene is...Ch. 14 - Prob. 28PCh. 14 - 14.29 As shown in Figure, mutations in the...Ch. 14 - How would you edit a specific nucleotide in a...Ch. 14 - Through a forward genetics screen in Arabidopsis...Ch. 14 - The CRISPR - Cas 9 complex directs the Cas 9...Ch. 14 - 14.33 Describe how enhancer screens can be used to...Ch. 14 - How might you use CRISPR - Cas 9 to create a large...
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- Each of the following describes a distinctive step in a genomic technology or an experimental design. Match the term for each description. An experimental design that looks at the offspring resulting from an F2 cross, where the parental generation have different phenotypes. Then, look for correlation between offspring phenotype and genotype. The intensity of fluorescence in each spot indicates the level of gene expression. Only one fluorescent nucleotide is added, which is complementary to the position of a known SNP. Familial Design [Choose ] RNA microarray GWAS Familial Design Quantitative Genetics Genomic resequencing RNA sequencing DNA microarray Genomic resequencing <arrow_forwardYou receive four strains of yeast in the mail, and the accompanying instructions state that each strain contains a single copy of transgene A. You grow the four strains and determine that only three strains express the protein product of transgene A. Further analysis reveals that transgene A is located at a different position in the yeast genome in each of the four strains. Provide a hypothesis to explain this result.arrow_forwardWhen the S.cerevisiae genome was sequenced and surveyed for possible genes, only about 40% of those genes had been previously identified in forward genetic screens. This left about 60% of predcited genes with no known function, leading some to dub the genes fun (function unknown) genes. a)As an approach to understanding the function of a certain fun gene, you wish to create a loss of function allele. How would you do this? b)You wish to know the physical location of the encoded protein product. How would you obtain such information?arrow_forward
- Suppose a researcher has three different Drosophila strains that have mutations in the bicoid gene called bicoid-A, bicoid-B, and bicoid-C; the wild type is designated bicoid +. To study these mutations, phenotypically normal female flies that are homozygous for the given bicoid mutation were obtained, and their oocytes were analyzed using a Northern blot to determine the size and/or amount of the bicoid mRNA and in situ hybridization to determine the bicoid mRNA location within the oocyte. A wild-type strain was also analyzed as a control. In both cases, the probe was complementary to the bicoid mRNA and the results are shown below. (Anterior is on the left; posterior is on the right.) Northern blot 1 2 - 3 4 In situ hybridization Wild type Lane 1. Wild type (bicoid*) Lane 2. bicoid-A Lane 3. bicoid-B Lane 4. bicoid-C bicoid-B bicoid-A bicoid-C Which mutation is likely to cause the embryo to develop two "anterior" ends? bicoid-B Obicoid-A bicoid-Carrow_forwardIn yeast, you have sequenced a piece of wild-type DNA and it clearly contains a gene, but you do not know what gene it is. Therefore, to investigate further, you would like to find out its mutant phenotype. How would you use the cloned wild-type gene to do so? Show your experimental steps clearlyarrow_forwardAfter much work the researcher maps the mutation in the mut1 plant to a locus on chromosome 2. The gene associated with the mut1 mutation is called COB1 (i.e. mutations in COB1 cause the mut1 phenotype.) The researcher extracts DNA and RNA from developing ears of wild type, mut1 and mut2 plants. From the DNA samples COB1 PCR primers are used to amplify the coding region of the COB1 gene (see DNA gel below). An RNA gel is used to examine the expression of COB1 all three plants (see RNA gel below). results in photo: Which of the following is TRUE about the COB1 gene. a. There is a deletion in the COB1 gene in the mut1 plants. b. COB1 is not transcribed in the mut2 plant. c. There is a deletion in the COB1 gene in the mut2 plants. d. COB1 is not transcribed in the mut1 plant.arrow_forward
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