Genetic Analysis: An Integrated Approach (2nd Edition)
Genetic Analysis: An Integrated Approach (2nd Edition)
2nd Edition
ISBN: 9780321948908
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 13, Problem 31P

Two NotI restriction enzymes cleave DNA on oppositesides of the Dbmgene in a species of yeast. A molecularprobe for Dbmdetects a DNA restriction fragment of 8 .5 kb in organisms that are wild type at Dbm. In a strain of yeast, a Ty 1 transposable genetic element mutates dbm. Ty 1 is 5 .6 kb in length.

In haploid yeast with this dbmmutation, what is thelength of the restriction fragment detected by the probefollowing NotI digestion?

What DNA-fragment sizes are detected in a diploidyeast strain that is heterozygous for wild-type andmutant alleles at dbm?

Insertion of Ty 1 into dbmcauses a loss-of-functionmutation. Explain why this is the case.

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The completely synthetic yeast chromosome Syn IIIcontains a loxP site in the 3′ UTR of every gene thatis potentially nonessential to yeast survival. As youwill recall from Chapter 6, loxP sites are targets ofsite-specific recombination. The researchers who constructed Syn III included these loxP sites as a way to“scramble” the chromosome, meaning that parts ofthe chromosome could easily be deleted or rearranged.The goal of these investigations is to drive the evolution of Syn III so as to define a minimal genome thatcan support the life of this organism. Outline the experiment the researchers would do to scramble Syn IIIin order to define a minimal genome.
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Genetic Analysis: An Integrated Approach (2nd Edition)

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