Brock Biology of Microorganisms (14th Edition)
14th Edition
ISBN: 9780321897398
Author: Michael T. Madigan, John M. Martinko, Kelly S. Bender, Daniel H. Buckley, David A. Stahl, Thomas Brock
Publisher: PEARSON
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Chapter 13, Problem 20RQ
Compare and contrast ferric iron reduction with reductive dechlorination in terms of (1) product of the reduction and (2) environmental significance.
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To purify a given enzyme from a crude extract that contains it, fractional precipitations, exchange chromatography are successively tested ionic and exclusion chromatography, with the results shown in the following table:
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A bacterial culture is grown using either octadecane (C18H38) or pentachlorophenol (C6HOCl5)) as the sole source of carbon and energy. The cell yield value is determined by dry weight analysis to be 1.49 for octadecane and 0.05 for pentachlorophenol.
Using either octadecane or pentachlorophenol, please describe the steps taken (and the final result) showing what percentage of the substrate carbon will be found as cell mass and as CO2?
Please show all calculations.
Chapter 13 Solutions
Brock Biology of Microorganisms (14th Edition)
Ch. 13.1 - What is the fundamental difference between an...Ch. 13.1 - Prob. 2MQCh. 13.1 - Why can phototrophic green bacteria grow at light...Ch. 13.2 - In which phototrophs are carotenoids found?...Ch. 13.2 - How does the structure of a phycobilin compare...Ch. 13.2 - Phycocyanin is blue-green. What color of light...Ch. 13.3 - What parallels exist in the processes of...Ch. 13.3 - What is reverse electron flow and why is it...Ch. 13.3 - Prob. 3MQCh. 13.4 - Differentiate between cyclic and noncyclic...
Ch. 13.4 - What is the key role of light energy in the...Ch. 13.4 - What evidence is there that anoxygenic and...Ch. 13.5 - Prob. 1MQCh. 13.5 - How much NADPH and ATP is required to make one...Ch. 13.5 - Contrast autotrophy in the following phototrophs:...Ch. 13.6 - Prob. 1MQCh. 13.6 - Prob. 2MQCh. 13.7 - What enzyme is required for hydrogen bacteria to...Ch. 13.7 - Why is reverse electron flow unnecessary in H2...Ch. 13.8 - Prob. 1MQCh. 13.8 - In terms of intermediates, how does the Sox system...Ch. 13.9 - Prob. 1MQCh. 13.9 - What is the function of rusticyanin and where is...Ch. 13.9 - How can Fe2+ be oxidized under anoxic conditions?Ch. 13.10 - Prob. 1MQCh. 13.10 - Prob. 2MQCh. 13.10 - Prob. 3MQCh. 13.11 - Prob. 1MQCh. 13.11 - Why is acetate formation in fermentation...Ch. 13.12 - How can homo- and heterofermentative metabolism be...Ch. 13.12 - Butanediol production leads to greater ethanol...Ch. 13.13 - Compare the mechanisms for energy conservation in...Ch. 13.13 - What type of substrates are fermented by...Ch. 13.13 - What are the substrates for the Clostridium...Ch. 13.14 - Why does Propionigenium modestum require sodium...Ch. 13.14 - Of what benefit is the organism Oxalobacter to...Ch. 13.14 - Prob. 3MQCh. 13.15 - Give an example of interspecies H2 transfer. Why...Ch. 13.15 - Why can a pure culture of Syntrophomonas grow on...Ch. 13.16 - How does aerobic respiration differ from anaerobic...Ch. 13.16 - Prob. 2MQCh. 13.17 - For Escherichia coli, why is more energy released...Ch. 13.17 - How do the products of NO3 reduction differ...Ch. 13.17 - Where is the dissimilative nitrate reductase found...Ch. 13.18 - How is SO42 converted to SO32 during dissimilative...Ch. 13.18 - Contrast the growth of Desulfovibrio on H2 versus...Ch. 13.18 - Give an example of sulfur disproportionation.Ch. 13.19 - Prob. 1MQCh. 13.19 - Prob. 2MQCh. 13.19 - Prob. 3MQCh. 13.20 - Which coenzymes function as C1 carriers in...Ch. 13.20 - In methanogens growing on H2 + CO2, how is carbon...Ch. 13.20 - How is ATP made in methanogenesis when the...Ch. 13.21 - Prob. 1MQCh. 13.21 - What is reductive dechlorination and why is it...Ch. 13.21 - How does anaerobic glucose catabolism differ in...Ch. 13.22 - How do monooxygenases differ in function from...Ch. 13.22 - What is the final product of catabolism of a...Ch. 13.22 - Prob. 3MQCh. 13.23 - When using CH4 as electron donor, why is...Ch. 13.23 - Prob. 2MQCh. 13.23 - In which two ways does the ribulose monophosphate...Ch. 13.24 - Prob. 1MQCh. 13.24 - How is hexane oxygenated during anoxic catabolism?Ch. 13.24 - Prob. 3MQCh. 13 - Prob. 1RQCh. 13 - Prob. 2RQCh. 13 - What accessory pigments are present in...Ch. 13 - Prob. 4RQCh. 13 - Prob. 5RQCh. 13 - Prob. 6RQCh. 13 - REVIEW QUESTIONS
7. What two enzymes are unique to...Ch. 13 - Prob. 8RQCh. 13 - Prob. 9RQCh. 13 - QWhich inorganic electron donors are used by the...Ch. 13 - Prob. 11RQCh. 13 - Define the term substrate-level phosphorylation:...Ch. 13 - Prob. 13RQCh. 13 - Prob. 14RQCh. 13 - Prob. 15RQCh. 13 - Prob. 16RQCh. 13 - Prob. 17RQCh. 13 - Prob. 18RQCh. 13 - Compare and contrast acetogens with methanogens in...Ch. 13 - Compare and contrast ferric iron reduction with...Ch. 13 - How do monooxygenases differ from dioxygenases in...Ch. 13 - Prob. 22RQCh. 13 - Prob. 23RQCh. 13 - Prob. 1AQCh. 13 - The growth rate of the phototrophic purple...Ch. 13 - Prob. 3AQCh. 13 - A fatty acid such as butyrate cannot be fermented...Ch. 13 - When methane is made from CO2 (plus H2) or from...
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