Genetics: From Genes to Genomes, 5th edition
5th Edition
ISBN: 9780073525310
Author: Leland H. Hartwell, Michael L. Goldberg, Janice A. Fischer, Leroy Hood, Charles F. Aquadro
Publisher: McGraw-Hill Education
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Chapter 13, Problem 12P
In two isolates (one is resistant to ampicillin, and the other is sensitive to ampicillin) of a new bacterium, you
found that genes encoding ampicillin resistance are being transferred into the sensitive strain
| a. | How would you know that gene transfer is taking place? |
| b. | To determine if the gene transfer is transformation or transduction, you treat the mixed culture of cells with DNase. Why would this treatment distinguish between these two modes of gene transfer? Describe the results predicted if the gene transfer is transformation versus transduction. |
| c. | To determine if the gene transfer involves transformation, conjugation, or transduction, you separate the ampicillin-resistant and ampicillin-sensitive strains by a membrane with pores that are smaller than the size of a bacterium, but larger than the sizes of bacteriophage or DNA fragments. If gene transfer is still observed, what mechanisms are possibly involved, and which are excluded? |
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Chapter 13 Solutions
Genetics: From Genes to Genomes, 5th edition
Ch. 13 - Choose the phrase from the right column that best...Ch. 13 - Prob. 2PCh. 13 - Now that the sequence of the entire E. coli K12...Ch. 13 - Bacterial genomes such as that of E. coli...Ch. 13 - List at least three features of eukaryotic genomes...Ch. 13 - Describe a mechanism by which a gene could move...Ch. 13 - High salt concentrations tend to cause protein...Ch. 13 - Prob. 8PCh. 13 - A liquid culture of E. coli at a concentration of...Ch. 13 - Pick out the medium i, ii, iii, or iv onto which...
Ch. 13 - Prob. 11PCh. 13 - In two isolates one is resistant to ampicillin,...Ch. 13 - E. coli cells usually have only one copy of the F...Ch. 13 - In E. coli, the genes purC and pyrB are located...Ch. 13 - DNA sequencing of the entire H. influenzae genome...Ch. 13 - Genes encoding toxins are often located on...Ch. 13 - a. You want to perform an interrupted-mating...Ch. 13 - Prob. 18PCh. 13 - One issue with interrupted-mating experiments such...Ch. 13 - Prob. 20PCh. 13 - Starting with an F- strain that was prototrophic...Ch. 13 - You can carry out matings between an Hfr and F...Ch. 13 - Genome sequences show that some pathogenic...Ch. 13 - Generalized and specialized transduction both...Ch. 13 - Prob. 25PCh. 13 - Prob. 26PCh. 13 - Prob. 27PCh. 13 - The sequence at one end of one strand of the...Ch. 13 - Prob. 29PCh. 13 - Suppose that you could obtain radioactively...
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