a. To determine: The DNA construct that can be used to exchange the yodA gene sequence with the jellyfish green fluorescent protein (GFP). Introduction: The genes are the sequence of nucleotides that are present on the chromosomes and encode for a specific protein that plays a crucial role in the functioning of the different processes in an organism. The gene is located at specific gene loci and can be structural or regulatory in nature.

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Answer 1

Question

Chapter 13, Problem 29P

Summary Introduction

a.

To determine:

The DNA construct that can be used to exchange the yodA gene sequence with the jellyfish green fluorescent protein (GFP).

Introduction:

The genes are the sequence of nucleotides that are present on the chromosomes and encode for a specific protein that plays a crucial role in the functioning of the different processes in an organism. The gene is located at specific gene loci and can be structural or regulatory in nature.

Summary Introduction

b.

To determine:

The non-functionality of yodA gene in E.coli.

Introduction:

Mitotic recombination is considered to be a rare event in which the genetic recombination takes place in the somatic cells. The mitotic homologous recombination occurs typically between the sister chromatids after replication and before cell division.

Summary Introduction

c.

To determine:

The approach to keep yodA functional in the presence of the green fluorescent gene.

Introduction:

Auxotrophs are the organisms that lack the nutrient or substrate that is needed for their growth. This organism cannot synthesize such substrate and can only gain it from an external source like a medium.

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After you feel comfortable with your counting method and identifying cells in the various stages of mitosis, use the four images below of whitefish blastula to count the cells in each stage until you reach 100 total cells, recording your data below in Data Table 1. (You may not need to use all four images. Stop counting when you reach 100 total cells.) After totaling the cells in each stage, calculate the percent of cells in each stage. (Divide total of stage by overall total of 100 and then multiply by 100 to obtain percentage.) Data Table 1Stage Totals PercentInterphase Mitosis: Prophase Metaphase Anaphase Telophase Cytokinesis Totals 100 100% To find the length of time whitefish blastula cells spend in each stage, multiply the percent (recorded as a decimal, in other words take the percent number and divide by 100) by 24 hours. (Example: If percent is 20%, then Time in Hours = .2 * 24 = 4.8) Record your data in Data…

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Answer 2

Question

Chapter 13, Problem 29P

Summary Introduction

a.

To determine:

The DNA construct that can be used to exchange the yodA gene sequence with the jellyfish green fluorescent protein (GFP).

Introduction:

The genes are the sequence of nucleotides that are present on the chromosomes and encode for a specific protein that plays a crucial role in the functioning of the different processes in an organism. The gene is located at specific gene loci and can be structural or regulatory in nature.

Summary Introduction

b.

To determine:

The non-functionality of yodA gene in E.coli.

Introduction:

Mitotic recombination is considered to be a rare event in which the genetic recombination takes place in the somatic cells. The mitotic homologous recombination occurs typically between the sister chromatids after replication and before cell division.

Summary Introduction

c.

To determine:

The approach to keep yodA functional in the presence of the green fluorescent gene.

Introduction:

Auxotrophs are the organisms that lack the nutrient or substrate that is needed for their growth. This organism cannot synthesize such substrate and can only gain it from an external source like a medium.

Expert Solution & Answer

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Answer 3

Question

Chapter 13, Problem 29P

Summary Introduction

a.

To determine:

The DNA construct that can be used to exchange the yodA gene sequence with the jellyfish green fluorescent protein (GFP).

Introduction:

The genes are the sequence of nucleotides that are present on the chromosomes and encode for a specific protein that plays a crucial role in the functioning of the different processes in an organism. The gene is located at specific gene loci and can be structural or regulatory in nature.

Summary Introduction

b.

To determine:

The non-functionality of yodA gene in E.coli.

Introduction:

Mitotic recombination is considered to be a rare event in which the genetic recombination takes place in the somatic cells. The mitotic homologous recombination occurs typically between the sister chromatids after replication and before cell division.

Summary Introduction

c.

To determine:

The approach to keep yodA functional in the presence of the green fluorescent gene.

Introduction:

Auxotrophs are the organisms that lack the nutrient or substrate that is needed for their growth. This organism cannot synthesize such substrate and can only gain it from an external source like a medium.

Expert Solution & Answer

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Answer 4

Question

Chapter 13, Problem 29P

Summary Introduction

a.

To determine:

The DNA construct that can be used to exchange the yodA gene sequence with the jellyfish green fluorescent protein (GFP).

Introduction:

The genes are the sequence of nucleotides that are present on the chromosomes and encode for a specific protein that plays a crucial role in the functioning of the different processes in an organism. The gene is located at specific gene loci and can be structural or regulatory in nature.

Summary Introduction

b.

To determine:

The non-functionality of yodA gene in E.coli.

Introduction:

Mitotic recombination is considered to be a rare event in which the genetic recombination takes place in the somatic cells. The mitotic homologous recombination occurs typically between the sister chromatids after replication and before cell division.

Summary Introduction

c.

To determine:

The approach to keep yodA functional in the presence of the green fluorescent gene.

Introduction:

Auxotrophs are the organisms that lack the nutrient or substrate that is needed for their growth. This organism cannot synthesize such substrate and can only gain it from an external source like a medium.

Expert Solution & Answer

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Answer 5

Chapter 13, Problem 29P

Summary Introduction

a.

To determine:

The DNA construct that can be used to exchange the yodA gene sequence with the jellyfish green fluorescent protein (GFP).

Introduction:

The genes are the sequence of nucleotides that are present on the chromosomes and encode for a specific protein that plays a crucial role in the functioning of the different processes in an organism. The gene is located at specific gene loci and can be structural or regulatory in nature.

Summary Introduction

b.

To determine:

The non-functionality of yodA gene in E.coli.

Introduction:

Mitotic recombination is considered to be a rare event in which the genetic recombination takes place in the somatic cells. The mitotic homologous recombination occurs typically between the sister chromatids after replication and before cell division.

Summary Introduction

c.

To determine:

The approach to keep yodA functional in the presence of the green fluorescent gene.

Introduction:

Auxotrophs are the organisms that lack the nutrient or substrate that is needed for their growth. This organism cannot synthesize such substrate and can only gain it from an external source like a medium.

Answer 6

Chapter 13, Problem 29P

Summary Introduction

a.

To determine:

The DNA construct that can be used to exchange the yodA gene sequence with the jellyfish green fluorescent protein (GFP).

Introduction:

The genes are the sequence of nucleotides that are present on the chromosomes and encode for a specific protein that plays a crucial role in the functioning of the different processes in an organism. The gene is located at specific gene loci and can be structural or regulatory in nature.

Answer 7

Chapter 13, Problem 29P

Summary Introduction

a.

To determine:

The DNA construct that can be used to exchange the yodA gene sequence with the jellyfish green fluorescent protein (GFP).

Introduction:

The genes are the sequence of nucleotides that are present on the chromosomes and encode for a specific protein that plays a crucial role in the functioning of the different processes in an organism. The gene is located at specific gene loci and can be structural or regulatory in nature.

Answer 8

Summary Introduction

b.

To determine:

The non-functionality of yodA gene in E.coli.

Introduction:

Mitotic recombination is considered to be a rare event in which the genetic recombination takes place in the somatic cells. The mitotic homologous recombination occurs typically between the sister chromatids after replication and before cell division.

Answer 9

Summary Introduction

b.

To determine:

The non-functionality of yodA gene in E.coli.

Introduction:

Mitotic recombination is considered to be a rare event in which the genetic recombination takes place in the somatic cells. The mitotic homologous recombination occurs typically between the sister chromatids after replication and before cell division.

Answer 10

Summary Introduction

c.

To determine:

The approach to keep yodA functional in the presence of the green fluorescent gene.

Introduction:

Auxotrophs are the organisms that lack the nutrient or substrate that is needed for their growth. This organism cannot synthesize such substrate and can only gain it from an external source like a medium.

Answer 11

Summary Introduction

c.

To determine:

The approach to keep yodA functional in the presence of the green fluorescent gene.

Introduction:

Auxotrophs are the organisms that lack the nutrient or substrate that is needed for their growth. This organism cannot synthesize such substrate and can only gain it from an external source like a medium.

Answer 12

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Answer 13

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Answer 14

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Answer 15

Ch. 13 - Choose the phrase from the right column that best...Ch. 13 - Prob. 2P Ch. 13 - Now that the sequence of the entire E. coli K12...Ch. 13 - Bacterial genomes such as that of E. coli...Ch. 13 - List at least three features of eukaryotic genomes...Ch. 13 - Describe a mechanism by which a gene could move...Ch. 13 - High salt concentrations tend to cause protein...Ch. 13 - Prob. 8P Ch. 13 - A liquid culture of E. coli at a concentration of...Ch. 13 - Pick out the medium i, ii, iii, or iv onto which...

Solution Summary: The author describes the DNA construct that can be used to exchange the yodA gene sequence with the jellyfish green fluorescent protein (GFP).

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