Genetics: From Genes to Genomes, 5th edition
Genetics: From Genes to Genomes, 5th edition
5th Edition
ISBN: 9780073525310
Author: Leland H. Hartwell, Michael L. Goldberg, Janice A. Fischer, Leroy Hood, Charles F. Aquadro
Publisher: McGraw-Hill Education
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Chapter 13, Problem 17P
a. You want to perform an interrupted-mating mapping with an E. coli Hfr strain that is Pyr, Met, Xyl Tyr, Arg, His, Mal, and Strs. Describe an appropriate bacterial strain to be used as the other partner in this mating.
b. In an Hfr × F cross, the pyrE gene enters the recipient in 5 minutes, but at this time point there are no exconjugants that are Met, Xyl, Tyr, Arg, His, or Mal . The mating is now allowed to proceed for 30 minutes and Pyr exconjugants are selected. Of the Pyr cells, 32% are Met, 94% are Xyl, 7% are Tyr, 59% are Arg, 0% are His, and 71% are Mal . What can you conclude about the order of the genes?
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